CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020280
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase II-associated protein 1 
Protein Synonyms/Alias
  
Gene Name
 RPAP1 
Gene Synonyms/Alias
 KIAA1403 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
537LARHDVIKGLLATSLubiquitination[1]
615GPTPSLYKVPCATAMubiquitination[1]
623VPCATAMKLLRVLASubiquitination[1]
780PCLRQTLKLLSRPEMubiquitination[1]
1337ARRSMLQKTWLLADEubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Required for interaction of the RNA polymerase II complex with acetylated histone H3. 
Sequence Annotation
 MOD_RES 72 72 Phosphoserine.
 MOD_RES 321 321 Phosphothreonine.
 MOD_RES 1116 1116 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1393 AA 
Protein Sequence
MLSRPKPGES EVDLLHFQSQ FLAAGAAPAV QLVKKGNRGG GDANSDRPPL QDHRDVVMLD 60
NLPDLPPALV PSPPKRARPS PGHCLPEDED PEERLRRHDQ HITAVLTKII ERDTSSVAVN 120
LPVPSGVAFP AVFLRSRDTQ GKSATSGKRS IFAQEIAARR IAEAKGPSVG EVVPNVGPPE 180
GAVTCETPTP RNQGCQLPGS SHSFQGPNLV TGKGLRDQEA EQEAQTIHEE NIARLQAMAP 240
EEILQEQQRL LAQLDPSLVA FLRSHSHTQE QTGETASEEQ RPGGPSANVT KEEPLMSAFA 300
SEPRKRDKLE PEAPALALPV TPQKEWLHMD TVELEKLHWT QDLPPVRRQQ TQERMQARFS 360
LQGELLAPDV DLPTHLGLHH HGEEAERAGY SLQELFHLTR SQVSQQRALA LHVLAQVISR 420
AQAGEFGDRL AGSVLSLLLD AGFLFLLRFS LDDRVDGVIA TAIRALRALL VAPGDEELLD 480
STFSWYHGAL TFPLMPSQED KEDEDEDEEC PAGKAKRKSP EEESRPPPDL ARHDVIKGLL 540
ATSLLPRLRY VLEVTYPGPA VVLDILAVLI RLARHSLESA TRVLECPRLI ETIVREFLPT 600
SWSPVGAGPT PSLYKVPCAT AMKLLRVLAS AGRNIAARLL SSFDLRSRLC RIIAEAPQEL 660
ALPPEEAEML STEALRLWAV AASYGQGGYL YRELYPVLMR ALQVVPRELS THPPQPLSMQ 720
RIASLLTLLT QLTLAAGSTP AETISDSAEA SLSATPSLVT WTQVSGLQPL VEPCLRQTLK 780
LLSRPEMWRA VGPVPVACLL FLGAYYQAWS QQPSSCPEDW LQDMQRLSEE LLLPLLSQPT 840
LGSLWDSLRH CSLLCNPLSC VPALEAPPSL VSLGCSGGCP RLSLAGSASP FPFLTALLSL 900
LNTLAQIHKG LCGQLAAILA APGLQNYFLQ CVAPGAAPHL TPFSAWALRH EYHLQYLALA 960
LAQKAAALQP LPATHAALYH GMALALLSRL LPGSEYLTHE LLLSCVFRLE FLPERTSGGP 1020
EAADFSDQLS LGSSRVPRCG QGTLLAQACQ DLPSIRNCYL THCSPARASL LASQALHRGE 1080
LQRVPTLLLP MPTEPLLPTD WPFLPLIRLY HRASDTPSGL SPTDTMGTAM RVLQWVLVLE 1140
SWRPQALWAV PPAARLARLM CVFLVDSELF RESPVQHLVA ALLAQLCQPQ VLPNLNLDCR 1200
LPGLTSFPDL YANFLDHFEA VSFGDHLFGA LVLLPLQRRF SVTLRLALFG EHVGALRALS 1260
LPLTQLPVSL ECYTVPPEDN LALLQLYFRT LVTGALRPRW CPVLYAVAVA HVNSFIFSQD 1320
PQSSDEVKAA RRSMLQKTWL LADEGLRQHL LHYKLPNSTL PEGFELYSQL PPLRQHYLQR 1380
LTSTVLQNGV SET 1393 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0032774; P:RNA biosynthetic process; IEA:GOC. 
Interpro
 IPR013929; RNA_pol_II_AP1_C.
 IPR013930; RNA_pol_II_AP1_N. 
Pfam
 PF08620; RPAP1_C
 PF08621; RPAP1_N 
SMART
  
PROSITE
  
PRINTS