CPLM-008121

Genbank Nucleotide ID

Mitogen-activated protein kinase 9

Protein Synonyms/Alias

MAP kinase 9; MAPK 9; SAPK-alpha; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; p54-alpha

Rattus norvegicus (Rat)

Position	Peptide	Type	References
250	TPSAEFMKKLQPTVR	ubiquitination	[1]

[1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]

Functional Description

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons (By similarity).

DOMAIN 26 321 Protein kinase.
NP_BIND 32 40 ATP (By similarity).
MOTIF 183 185 TXY.
ACT_SITE 151 151 Proton acceptor (By similarity).
BINDING 55 55 ATP (By similarity).
MOD_RES 183 183 Phosphothreonine; by MAP2K7 (By
MOD_RES 185 185 Phosphotyrosine; by MAP2K4 (By

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:MGI.
GO:0005739; C:mitochondrion; IDA:RGD.
GO:0005634; C:nucleus; IDA:RGD.
GO:0005524; F:ATP binding; IDA:RGD.
GO:0008656; F:cysteine-type endopeptidase activator activity involved in apoptotic process; IMP:RGD.
GO:0004705; F:JUN kinase activity; IDA:RGD.
GO:0008134; F:transcription factor binding; IDA:RGD.
GO:0071363; P:cellular response to growth factor stimulus; IEP:RGD.
GO:0071347; P:cellular response to interleukin-1; IEP:RGD.
GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
GO:0071356; P:cellular response to tumor necrosis factor; IEP:RGD.
GO:0034644; P:cellular response to UV; IMP:RGD.
GO:0007417; P:central nervous system development; IEP:RGD.
GO:0031175; P:neuron projection development; IMP:RGD.
GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
GO:2001235; P:positive regulation of apoptotic signaling pathway; IEA:Compara.
GO:0010770; P:positive regulation of cell morphogenesis involved in differentiation; IMP:RGD.
GO:0032722; P:positive regulation of chemokine production; IMP:RGD.
GO:0010744; P:positive regulation of macrophage derived foam cell differentiation; IEA:Compara.
GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IMP:RGD.
GO:0051770; P:positive regulation of nitric-oxide synthase biosynthetic process; IMP:RGD.
GO:0031394; P:positive regulation of prostaglandin biosynthetic process; IMP:RGD.
GO:0032308; P:positive regulation of prostaglandin secretion; IMP:RGD.
GO:0001934; P:positive regulation of protein phosphorylation; IMP:RGD.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:RGD.
GO:0006626; P:protein targeting to mitochondrion; IEP:RGD.
GO:0046328; P:regulation of JNK cascade; IMP:RGD.
GO:0031396; P:regulation of protein ubiquitination; IMP:RGD.
GO:0001836; P:release of cytochrome c from mitochondria; IMP:RGD.
GO:0014075; P:response to amine stimulus; IEP:RGD.
GO:0046686; P:response to cadmium ion; IEA:Compara.
GO:0042493; P:response to drug; IMP:RGD.
GO:0009612; P:response to mechanical stimulus; IEP:RGD.
GO:0009636; P:response to toxic substance; IEP:RGD.

IPR011009; Kinase-like_dom.
IPR003527; MAP_kinase_CS.
IPR008351; MAPK_JNK.
IPR000719; Prot_kinase_cat_dom.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

PS01351; MAPK
PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST

PR01772; JNKMAPKINASE.