CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009878
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosol aminopeptidase 
Protein Synonyms/Alias
 Aminopeptidase A/I; Leucine aminopeptidase; LAP; Leucyl aminopeptidase 
Gene Name
 pepA 
Gene Synonyms/Alias
 carP; xerB; b4260; JW4217 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
6**MEFSVKSGSPEKQacetylation[1]
12VKSGSPEKQRSACIVacetylation[1]
37PIAEQLDKISDGYISacetylation[1]
55RRGELEGKPGQTLLLacetylation[1]
80ILLIGCGKERELDERacetylation[1]
90ELDERQYKQVIQKTIacetylation[1]
134RQAVETAKETLYSFDacetylation[1, 2, 3]
216QLADSYSKNVITRVIacetylation[1, 3]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Required for plasmid ColE1 site-specific recombination but not in its aminopeptidase activity. Could act as a structural component of the putative nucleoprotein complex in which the Xer recombination reaction takes place. 
Sequence Annotation
 ACT_SITE 282 282 Potential.
 ACT_SITE 356 356 Potential.
 METAL 270 270 Manganese 2 (Probable).
 METAL 275 275 Manganese 1 (Probable).
 METAL 275 275 Manganese 2 (Probable).
 METAL 293 293 Manganese 2 (Probable).
 METAL 352 352 Manganese 1 (Probable).
 METAL 354 354 Manganese 1 (Probable).
 METAL 354 354 Manganese 2 (Probable).  
Keyword
 3D-structure; Aminopeptidase; Complete proteome; Direct protein sequencing; Hydrolase; Manganese; Metal-binding; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG ELEGKPGQTL 60
LLHHVPNVLS ERILLIGCGK ERELDERQYK QVIQKTINTL NDTGSMEAVC FLTELHVKGR 120
NNYWKVRQAV ETAKETLYSF DQLKTNKSEP RRPLRKMVFN VPTRRELTSG ERAIQHGLAI 180
AAGIKAAKDL GNMPPNICNA AYLASQARQL ADSYSKNVIT RVIGEQQMKE LGMHSYLAVG 240
QGSQNESLMS VIEYKGNASE DARPIVLVGK GLTFDSGGIS IKPSEGMDEM KYDMCGAAAV 300
YGVMRMVAEL QLPINVIGVL AGCENMPGGR AYRPGDVLTT MSGQTVEVLN TDAEGRLVLC 360
DVLTYVERFE PEAVIDVATL TGACVIALGH HITGLMANHN PLAHELIAAS EQSGDRAWRL 420
PLGDEYQEQL ESNFADMANI GGRPGGAITA GCFLSRFTRK YNWAHLDIAG TAWRSGKAKG 480
ATGRPVALLA QFLLNRAGFN GEE 503 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:HAMAP.
 GO:0004177; F:aminopeptidase activity; IDA:EcoliWiki.
 GO:0003677; F:DNA binding; IDA:EcoliWiki.
 GO:0001073; F:DNA binding transcription antitermination factor activity; IMP:EcoliWiki.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP.
 GO:0043171; P:peptide catabolic process; IMP:EcoliWiki.
 GO:0006276; P:plasmid maintenance; IMP:EcoliWiki.
 GO:0042150; P:plasmid recombination; IMP:EcoliWiki.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IDA:EcoCyc. 
Interpro
 IPR011356; Leucine_aapep/pepB.
 IPR000819; Peptidase_M17_C.
 IPR023042; Peptidase_M17_leu_NH2_pept.
 IPR008283; Peptidase_M17_N. 
Pfam
 PF00883; Peptidase_M17
 PF02789; Peptidase_M17_N 
SMART
  
PROSITE
 PS00631; CYTOSOL_AP 
PRINTS
 PR00481; LAMNOPPTDASE.