CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022968
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 12 member 6 
Protein Synonyms/Alias
 Electroneutral potassium-chloride cotransporter 3; K-Cl cotransporter 3 
Gene Name
 SLC12A6 
Gene Synonyms/Alias
 KCC3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30EPSLADIKARIQDSDubiquitination[1, 2, 3]
80EGDEYFDKNLALFEEubiquitination[3]
549RLLQAIAKDNIIPFLubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. 
Sequence Annotation
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 1007 1007 Phosphoserine (By similarity).
 MOD_RES 1032 1032 Phosphoserine.
 CARBOHYD 379 379 N-linked (GlcNAc...) (Potential).
 CARBOHYD 398 398 N-linked (GlcNAc...) (Potential).
 CARBOHYD 411 411 N-linked (GlcNAc...) (Potential).
 CARBOHYD 428 428 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative promoter usage; Alternative splicing; Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Polymorphism; Potassium; Potassium transport; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1150 AA 
Protein Sequence
MPHFTVTKVE DPEEGAAASI SQEPSLADIK ARIQDSDEPD LSQNSITGEH SQLLDDGHKK 60
ARNAYLNNSN YEEGDEYFDK NLALFEEEMD TRPKVSSLLN RMANYTNLTQ GAKEHEEAEN 120
ITEGKKKPTK TPQMGTFMGV YLPCLQNIFG VILFLRLTWV VGTAGVLQAF AIVLICCCCT 180
MLTAISMSAI ATNGVVPAGG SYFMISRALG PEFGGAVGLC FYLGTTFAAA MYILGAIEIF 240
LVYIVPRAAI FHSDDALKES AAMLNNMRVY GTAFLVLMVL VVFIGVRYVN KFASLFLACV 300
IVSILAIYAG AIKSSFAPPH FPVCMLGNRT LSSRHIDVCS KTKEINNMTV PSKLWGFFCN 360
SSQFFNATCD EYFVHNNVTS IQGIPGLASG IITENLWSNY LPKGEIIEKP SAKSSDVLGS 420
LNHEYVLVDI TTSFTLLVGI FFPSVTGIMA GSNRSGDLKD AQKSIPIGTI LAILTTSFVY 480
LSNVVLFGAC IEGVVLRDKF GDAVKGNLVV GTLSWPSPWV IVIGSFFSTC GAGLQSLTGA 540
PRLLQAIAKD NIIPFLRVFG HSKANGEPTW ALLLTAAIAE LGILIASLDL VAPILSMFFL 600
MCYLFVNLAC ALQTLLRTPN WRPRFRYYHW ALSFMGMSIC LALMFISSWY YAIVAMVIAG 660
MIYKYIEYQG AEKEWGDGIR GLSLSAARFA LLRLEEGPPH TKNWRPQLLV LLKLDEDLHV 720
KHPRLLTFAS QLKAGKGLTI VGSVIVGNFL ENYGEALAAE QTIKHLMEAE KVKGFCQLVV 780
AAKLREGISH LIQSCGLGGM KHNTVVMGWP NGWRQSEDAR AWKTFIGTVR VTTAAHLALL 840
VAKNISFFPS NVEQFSEGNI DVWWIVHDGG MLMLLPFLLK QHKVWRKCSI RIFTVAQLED 900
NSIQMKKDLA TFLYHLRIEA EVEVVEMHDS DISAYTYERT LMMEQRSQML RHMRLSKTER 960
DREAQLVKDR NSMLRLTSIG SDEDEETETY QEKVHMTWTK DKYMASRGQK AKSMEGFQDL 1020
LNMRPDQSNV RRMHTAVKLN EVIVNKSHEA KLVLLNMPGP PRNPEGDENY MEFLEVLTEG 1080
LERVLLVRGG GSEVITIYS 1099 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0015379; F:potassium:chloride symporter activity; IDA:UniProtKB.
 GO:0001525; P:angiogenesis; NAS:UniProtKB.
 GO:0071477; P:cellular hypotonic salinity response; IDA:UniProtKB.
 GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW. 
Interpro
 IPR004841; AA-permease_dom.
 IPR018491; K/Cl_cotranspt_1/3.
 IPR000076; KCL_cotranspt.
 IPR004842; Na/K/Cl_cotransptS. 
Pfam
 PF00324; AA_permease
 PF03522; KCl_Cotrans_1 
SMART
  
PROSITE
  
PRINTS
 PR01081; KCLTRNSPORT.