CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-tubulin complex component 4 
Protein Synonyms/Alias
 GCP-4; hGCP4; Gamma-ring complex protein 76 kDa; h76p; hGrip76 
Gene Name
 TUBGCP4 
Gene Synonyms/Alias
 76P; GCP4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
233GIGGLTGKQLRELQDubiquitination[1, 2]
255NMLAPSLKQFSLRVEubiquitination[2]
301TRKGSILKNQEDTFAubiquitination[2]
315AAELHRLKQQPLFSLubiquitination[1, 2, 3]
358LGQLKIIKDFYLLGRubiquitination[4, 5]
506QMQRKHLKSNQTDAIubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. 
Sequence Annotation
  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Microtubule; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 667 AA 
Protein Sequence
MIHELLLALS GYPGSIFTWN KRSGLQVSQD FPFLHPSETS VLNRLCRLGT DYIRFTEFIE 60
QYTGHVQQQD HHPSQQGQGG LHGIYLRAFC TGLDSVLQPY RQALLDLEQE FLGDPHLSIS 120
HVNYFLDQFQ LLFPSVMVVV EQIKSQKIHG CQILETVYKH SCGGLPPVRS ALEKILAVCH 180
GVMYKQLSAW MLHGLLLDQH EEFFIKQGPS SGNVSAQPEE DEEDLGIGGL TGKQLRELQD 240
LRLIEEENML APSLKQFSLR VEILPSYIPV RVAEKILFVG ESVQMFENQN VNLTRKGSIL 300
KNQEDTFAAE LHRLKQQPLF SLVDFEQVVD RIRSTVAEHL WKLMVEESDL LGQLKIIKDF 360
YLLGRGELFQ AFIDTAQHML KTPPTAVTEH DVNVAFQQSA HKVLLDDDNL LPLLHLTIEY 420
HGKEHKADAT QAREGPSRET SPREAPASGW AALGLSYKVQ WPLHILFTPA VLEKYNVVFK 480
YLLSVRRVQA ELQHCWALQM QRKHLKSNQT DAIKWRLRNH MAFLVDNLQY YLQVDVLESQ 540
FSQLLHQINS TRDFESIRLA HDHFLSNLLA QSFILLKPVF HCLNEILDLC HSFCSLVSQN 600
LGPLDERGAA QLSILVKGFS RQSSLLFKIL SSVRNHQINS DLAQLLLRLD YNKYYTQAGG 660
TLGSFGM 667 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008274; C:gamma-tubulin ring complex; NAS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0000922; C:spindle pole; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; NAS:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007020; P:microtubule nucleation; NAS:UniProtKB.
 GO:0006461; P:protein complex assembly; NAS:ProtInc. 
Interpro
 IPR007259; Spc97_Spc98. 
Pfam
 PF04130; Spc97_Spc98 
SMART
  
PROSITE
  
PRINTS