UniProt Accession

 MLC1_YEAST; P53141; D6VU40 

Genbank Protein ID

 X97644; Z72628; BK006941 

Genbank Nucleotide ID

 CAA66246.1; CAA96813.1; DAA08001.1 

Protein Name

 Myosin light chain 1 

Protein Synonyms/Alias

 Calmodulin-like myosin light chain MLC1; Myosin-2 light chain 

Gene Name

 MLC1 

Gene Synonyms/Alias

 YGL106W; G3080 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
8	MSATRANKDIFTLFD	acetylation	[1]
16	DIFTLFDKKGQGAIA	acetylation	[1]
95	KAFQVFDKESTGKVS	acetylation	[1]
116	MLTGLGEKLTDAEVD	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2. 

Sequence Annotation

 DOMAIN 2 37 EF-hand 1.
 DOMAIN 81 116 EF-hand 2.
 DOMAIN 117 149 EF-hand 3.  

Keyword

 3D-structure; Calcium; Cell cycle; Cell division; Complete proteome; Metal-binding; Motor protein; Myosin; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 149 AA 

Protein Sequence

Gene Ontology

 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0031982; C:vesicle; IDA:SGD.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0031489; F:myosin V binding; IDA:SGD.
 GO:0000915; P:cytokinesis, actomyosin contractile ring assembly; IMP:SGD.
 GO:0008104; P:protein localization; IMP:SGD.
 GO:0006903; P:vesicle targeting; IMP:SGD. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS