UniProt Accession

 NDKA_MOUSE; P15532 

Genbank Protein ID

 M35970; M65037; U85511; AF033377; BC005629 

Genbank Nucleotide ID

 AAA39826.1; AAA63391.1; AAB42080.1; AAB87689.1; AAH05629.1 

Protein Name

 Nucleoside diphosphate kinase A 

Protein Synonyms/Alias

 NDK A; NDP kinase A; Metastasis inhibition factor NM23; NDPK-A; Tumor metastatic process-associated protein; nm23-M1 

Gene Name

 Nme1 

Gene Synonyms/Alias

 Nm23 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
124	IHGSDSVKSAEKEIS	acetylation	[1, 2, 3, 4]
124	IHGSDSVKSAEKEIS	ubiquitination	[5]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination (By similarity). 

Sequence Annotation

 ACT_SITE 118 118 Pros-phosphohistidine intermediate.
 BINDING 12 12 ATP (By similarity).
 BINDING 60 60 ATP (By similarity).
 BINDING 88 88 ATP (By similarity).
 BINDING 94 94 ATP (By similarity).
 BINDING 105 105 ATP (By similarity).
 BINDING 115 115 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 CROSSLNK 100 100 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Differentiation; Direct protein sequencing; Endocytosis; Isopeptide bond; Kinase; Magnesium; Metal-binding; Neurogenesis; Nucleotide metabolism; Nucleotide-binding; Nucleus; Reference proteome; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004536; F:deoxyribonuclease activity; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:Compara.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0004550; F:nucleoside diphosphate kinase activity; IEA:EC.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IEA:Compara.
 GO:0003697; F:single-stranded DNA binding; IEA:Compara.
 GO:0035690; P:cellular response to drug; IEA:Compara.
 GO:0071398; P:cellular response to fatty acid; IEA:Compara.
 GO:0071333; P:cellular response to glucose stimulus; IEA:Compara.
 GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
 GO:0021766; P:hippocampus development; IEA:Compara.
 GO:0007595; P:lactation; IMP:MGI.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0002762; P:negative regulation of myeloid leukocyte differentiation; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
 GO:0010976; P:positive regulation of neuron projection development; IEA:Compara.
 GO:0014075; P:response to amine stimulus; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0033574; P:response to testosterone stimulus; IEA:Compara.
 GO:0006228; P:UTP biosynthetic process; IEA:InterPro. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.