CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007945
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-actin-capping protein subunit beta 
Protein Synonyms/Alias
 CapZ beta 
Gene Name
 CAPZB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53PLKIARDKVVGKDYLubiquitination[1]
57ARDKVVGKDYLLCDYubiquitination[1]
78YRSPWSNKYDPPLEDubiquitination[1, 2, 3]
95MPSARLRKLEVEANNubiquitination[1, 2, 3]
159HVVEVQEKSSGRTAHubiquitination[1]
199SLTRQMEKDETVSDCubiquitination[1]
223LVEDMENKIRSTLNEubiquitination[1]
235LNEIYFGKTKDIVNGacetylation[4]
235LNEIYFGKTKDIVNGubiquitination[1, 2, 3]
237EIYFGKTKDIVNGLRubiquitination[1]
256IPDNQKFKQLQRELSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 182 182 Phosphoserine (By similarity).
 MOD_RES 235 235 N6-acetyllysine.  
Keyword
 Acetylation; Actin capping; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 277 AA 
Protein Sequence
MSDQQLDCAL DLMRRLPPQQ IEKNLSDLID LVPSLCEDLL SSVDQPLKIA RDKVVGKDYL 60
LCDYNRDGDS YRSPWSNKYD PPLEDGAMPS ARLRKLEVEA NNAFDQYRDL YFEGGVSSVY 120
LWDLDHGFAG VILIKKAGDG SKKIKGCWDS IHVVEVQEKS SGRTAHYKLT STVMLWLQTN 180
KSGSGTMNLG GSLTRQMEKD ETVSDCSPHI ANIGRLVEDM ENKIRSTLNE IYFGKTKDIV 240
NGLRSIDAIP DNQKFKQLQR ELSQVLTQRQ IYIQPDN 277 
Gene Ontology
 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008290; C:F-actin capping protein complex; TAS:ProtInc.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0030017; C:sarcomere; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; IDA:UniProtKB.
 GO:0003779; F:actin binding; TAS:ProtInc.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0051693; P:actin filament capping; IEA:UniProtKB-KW.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0006928; P:cellular component movement; TAS:UniProtKB.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0022604; P:regulation of cell morphogenesis; IMP:UniProtKB. 
Interpro
 IPR019771; F-actin_capping_bsu_CS.
 IPR001698; WASH_F-actin_cap_beta. 
Pfam
 PF01115; F_actin_cap_B 
SMART
  
PROSITE
 PS00231; F_ACTIN_CAPPING_BETA 
PRINTS
 PR00192; FACTINCAPB.