CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015923
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear fragile X mental retardation-interacting protein 2 
Protein Synonyms/Alias
 82 kDa FMRP-interacting protein; 82-FIP; Cell proliferation-inducing gene 1 protein; FMRP-interacting protein 2 
Gene Name
 NUFIP2 
Gene Synonyms/Alias
 KIAA1321; PIG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
109GEREISLKNLSSDEAubiquitination[1, 2]
136QVVDTSLKQTVKANTubiquitination[2]
140TSLKQTVKANTFGKAacetylation[3]
262KQGLETFKPDYSEQKacetylation[4]
569PKAYELEKRTSPQVLubiquitination[5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Binds RNA. 
Sequence Annotation
 MOD_RES 87 87 Phosphothreonine.
 MOD_RES 112 112 Phosphoserine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 218 218 Phosphotyrosine.
 MOD_RES 219 219 Phosphothreonine.
 MOD_RES 220 220 Phosphothreonine.
 MOD_RES 376 376 Phosphoserine.
 MOD_RES 571 571 Phosphothreonine.
 MOD_RES 572 572 Phosphoserine.
 MOD_RES 629 629 Phosphoserine.
 MOD_RES 633 633 Phosphothreonine.
 MOD_RES 637 637 Phosphoserine.
 MOD_RES 652 652 Phosphoserine.
 MOD_RES 655 655 Phosphoserine.
 MOD_RES 692 692 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 695 AA 
Protein Sequence
MEEKPGQPQP QHHHSHHHPH HHPQQQQQQP HHHHHYYFYN HSHNHHHHHH HQQPHQYLQH 60
GAEGSPKAQP KPLKHEQKHT LQQHQETPKK KTGYGELNGN AGEREISLKN LSSDEATNPI 120
SRVLNGNQQV VDTSLKQTVK ANTFGKAGIK TKNFIQKNSM DKKNGKSYEN KSGENQSVDK 180
SDTIPIPNGV VTNNSGYITN GYMGKGADND GSGSESGYTT PKKRKARRNS AKGCENLNIV 240
QDKIMQQETS VPTLKQGLET FKPDYSEQKG NRVDGSKPIW KYETGPGGTS RGKPAVGDML 300
RKSSDSKPGV SSKKFDDRPK GKHASAVASK EDSWTLFKPP PVFPVDNSSA KIVPKISYAS 360
KVKENLNKTI QNSSVSPTSS SSSSSSTGET QTQSSSRLSQ VPMSALKSVT SANFSNGPVL 420
AGTDGNVYPP GGQPLLTTAA NTLTPISSGT DSVLQDMSLT SAAVEQIKTS LFIYPSNMQT 480
MLLSTAQVDL PSQTDQQNLG DIFQNQWGLS FINEPSAGPE TVTGKSSEHK VMEVTFQGEY 540
PATLVSQGAE IIPSGTEHPV FPKAYELEKR TSPQVLGSIL KSGTTSESGA LSLEPSHIGD 600
LQKADTSSQG ALVFLSKDYE IESQNPLASP TNTLLGSAKE QRYQRGLERN DSWGSFDLRA 660
AIVYHTKEME SIWNLQKQDP KRIITYNEAM DSPDQ 695 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0042788; C:polysomal ribosome; IDA:HGNC.
 GO:0003723; F:RNA binding; IDA:HGNC. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS