CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024124
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General vesicular transport factor p115 
Protein Synonyms/Alias
 Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein 
Gene Name
 Uso1 
Gene Synonyms/Alias
 Vdp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
25TEAETIQKLCDRVASubiquitination[1]
202RSNGAIQKIVAFENAubiquitination[1]
580GKENFIEKLGFISKHacetylation[2]
580GKENFIEKLGFISKHubiquitination[1]
626ELEGVITKAIYKSSEacetylation[2, 3]
626ELEGVITKAIYKSSEubiquitination[1]
643KKEEEVKKTLEQHDNubiquitination[1]
698ASQIQQHKDQYNLLKubiquitination[1]
705KDQYNLLKVQLGKDNubiquitination[1]
710LLKVQLGKDNHHQGSubiquitination[1]
743REEIEELKSQQALLQubiquitination[1]
842SEHVSAAKTTDVEGRubiquitination[1]
858SALLQETKELKNEIKacetylation[3]
876EERTAIQKQLDSSNSubiquitination[1]
894ILQTEKDKLDLEVTDubiquitination[1]
919LLADQDQKILSLKSKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity). 
Sequence Annotation
 REPEAT 20 60 ARM 1.
 REPEAT 61 121 ARM 2.
 REPEAT 123 163 ARM 3.
 REPEAT 166 207 ARM 4.
 REPEAT 208 253 ARM 5.
 REPEAT 255 310 ARM 6.
 REPEAT 311 354 ARM 7.
 REPEAT 363 408 ARM 8.
 REPEAT 420 459 ARM 9.
 REPEAT 473 513 ARM 10.
 REPEAT 518 571 ARM 11.
 REPEAT 573 630 ARM 12.
 REGION 1 637 Globular head.
 MOD_RES 50 50 Phosphoserine (By similarity).
 MOD_RES 202 202 N6-acetyllysine (By similarity).
 MOD_RES 940 940 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 959 AA 
Protein Sequence
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 60
AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE NSTRQSEDLG SQFTEIFIKQ 120
PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSR LMDLLADSRE 180
IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 240
KTNNSNQNFF KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 300
SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 360
PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 420
AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 480
ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 540
CALLLGISIY FNDNSLENYT KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN 600
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 660
EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ YNLLKVQLGK DNHHQGSHGD 720
GAQVNGIQPE EISRLREEIE ELKSQQALLQ GQLAEKDSLI ENLKSSQASG MSEQASATCP 780
PRDPEQVAEL KQELTALKSQ LCSQSLEITR LQTENCELLQ RAETLAKSVP VEGESEHVSA 840
AKTTDVEGRL SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLDLEVT 900
DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEI DDGDKDQDI 959 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0008565; F:protein transporter activity; IDA:MGI.
 GO:0006886; P:intracellular protein transport; IDA:MGI.
 GO:0045056; P:transcytosis; IEA:Compara.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR006955; Uso1_p115_C.
 IPR024095; Vesicle_P115-like.
 IPR006953; Vesicle_Uso1_P115_head. 
Pfam
 PF04871; Uso1_p115_C
 PF04869; Uso1_p115_head 
SMART
  
PROSITE
 PS50176; ARM_REPEAT 
PRINTS