UniProt Accession

 CFAD_HUMAN; P00746; B4DV76; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4 

Genbank Protein ID

 AJ313463; AK300963; CH471139; BC034529; BC040146; BC051001; BC057807; M84526 

Genbank Nucleotide ID

 CAC48304.1; BAG62588.1; EAW69588.1; AAH34529.1; AAH40146.1; AAH51001.1; AAH57807.1; AAA35527.1 

Protein Name

 Complement factor D 

Protein Synonyms/Alias

 Adipsin; C3 convertase activator; Properdin factor D 

Gene Name

 CFD 

Gene Synonyms/Alias

 DF; PFD 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
92	LSQPEPSKRLYDVLR	ubiquitination	[1, 2, 3, 4]
123	LLLQLSEKATLGPAV	ubiquitination	[2]
205	SNRRDSCKGDSGGPL	ubiquitination	[3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. 

Sequence Annotation

 DOMAIN 26 253 Peptidase S1.
 ACT_SITE 66 66 Charge relay system.
 ACT_SITE 114 114 Charge relay system.
 ACT_SITE 208 208 Charge relay system.
 DISULFID 51 67
 DISULFID 148 214
 DISULFID 179 195
 DISULFID 204 229  

Keyword

 3D-structure; Complement alternate pathway; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Hydrolase; Immunity; Innate immunity; Polymorphism; Protease; Reference proteome; Secreted; Serine protease; Signal; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 253 AA 

Protein Sequence

Gene Ontology

 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0031093; C:platelet alpha granule lumen; TAS:Reactome.
 GO:0004252; F:serine-type endopeptidase activity; TAS:ProtInc.
 GO:0006957; P:complement activation, alternative pathway; TAS:Reactome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0002576; P:platelet degranulation; TAS:Reactome.
 GO:0006508; P:proteolysis; TAS:ProtInc. 

Interpro

 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR009003; Trypsin-like_Pept_dom. 

Pfam

 PF00089; Trypsin 

SMART

 SM00020; Tryp_SPc 

PROSITE

 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 

PRINTS

 PR00722; CHYMOTRYPSIN.