CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021542
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RecQ-mediated genome instability protein 1 
Protein Synonyms/Alias
 BLM-associated protein of 75 kDa; BLAP75; FAAP75 
Gene Name
 RMI1 
Gene Synonyms/Alias
 C9orf76 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130TPKPWEAKPSRMLMLubiquitination[1, 2]
183RLGVLLLKPENVKVLubiquitination[1]
205LEEYAQEKVLARLIGubiquitination[1, 3]
334QKEQMETKELQPLTFubiquitination[1]
376GNNNWSEKNVSEQMTubiquitination[1]
387EQMTNEDKSFGCPSVubiquitination[1]
441DSHSLNNKILNREVVubiquitination[1]
453EVVNYVQKRNSQISNubiquitination[1, 4]
566PEMKQSKKDPLQYQKubiquitination[1]
573KDPLQYQKFLEGLQKubiquitination[1]
580KFLEGLQKCQRDLIDubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability. 
Sequence Annotation
 MOD_RES 225 225 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 625 AA 
Protein Sequence
MNVTSIALRA ETWLLAAWHV KVPPMWLEAC INWIQEENNN VNLSQAQMNK QVFEQWLLTD 60
LRDLEHPLLP DGILEIPKGE LNGFYALQIN SLVDVSQPAY SQIQKLRGKN TTNDLVTAEA 120
QVTPKPWEAK PSRMLMLQLT DGIVQIQGME YQPIPILHSD LPPGTKILIY GNISFRLGVL 180
LLKPENVKVL GGEVDALLEE YAQEKVLARL IGEPDLVVSV IPNNSNENIP RVTDVLDPAL 240
GPSDEELLAS LDENDELTAN NDTSSERCFT TGSSSNTIPT RQSSFEPEFV ISPRPKEEPS 300
NLSIHVMDGE LDDFSLEEAL LLEETVQKEQ METKELQPLT FNRNADRSIE RFSHNPNTTN 360
NFSLTCKNGN NNWSEKNVSE QMTNEDKSFG CPSVRDQNRS IFSVHCNVPL AHDFTNKEKN 420
LETDNKIKQT SSSDSHSLNN KILNREVVNY VQKRNSQISN ENDCNLQSCS LRSSENSINL 480
SIAMDLYSPP FVYLSVLMAS KPKEVTTVKV KAFIVTLTGN LSSSGGIWSI TAKVSDGTAY 540
LDVDFVDEIL TSLIGFSVPE MKQSKKDPLQ YQKFLEGLQK CQRDLIDLCC LMTISFNPSL 600
SKAMVLALQD VNMEHLENLK KRLNK 625 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW. 
Interpro
 IPR013894; DUF1767. 
Pfam
 PF08585; DUF1767 
SMART
  
PROSITE
  
PRINTS