CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021080
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional protein NCOAT 
Protein Synonyms/Alias
 Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; Protein O-GlcNAcase; Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA; Histone acetyltransferase; HAT 
Gene Name
 Mgea5 
Gene Synonyms/Alias
 Hexc; Kiaa0679 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
278YLRTVGEKLLPGIEVubiquitination[1]
335RLFLGPYKGRSTELIubiquitination[1]
761TIRPYFPKDEASVYKubiquitination[1]
768KDEASVYKICREMYDubiquitination[1]
907ACLLSSLKANGSRGAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity (By similarity). Acetylates 'Lys-8' of histone H4. 
Sequence Annotation
 REGION 583 916 Histone acetyltransferase activity (By
 REGION 695 814 Required for histone H4 binding.
 ACT_SITE 175 175 Nucleophile; for O-GlcNAcase activity.
 ACT_SITE 177 177 Proton donor; for O-GlcNAcase activity.
 MOD_RES 364 364 Phosphoserine.
 DISULFID 777 793  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Glycosidase; Hydrolase; Multifunctional enzyme; Nucleus; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MVRPRVWRSS RRVASQNGLR AFGPTDRGRR GAVAGGRRMV QKESQAALEE RESERNANPA 60
AASGASLEQS VAPAPGEDNP SGAGAAAVVG AAGGARRFLC GVVEGFYGRP WVMEQRKELF 120
RRLQKWELNT YLYAPKDDYK HRMFWREMYS VEEAEQLMTL ISAAREYEIE FIYAISPGLD 180
ITFSNPKEVS TLKRKLDQVS QFGCRSFALL FDDIDHNMCA ADKEVFSSFA HAQVSITNEI 240
YQYLGEPETF LFCPTEYCGT FCYPNVSQSP YLRTVGEKLL PGIEVLWTGP KVVSKEIPVE 300
SIEEVSKIIK RAPVIWDNIH ANDYDQKRLF LGPYKGRSTE LIPRLKGVLT NPNCEFEANY 360
VAIHTLATWY KSNMNGVRKD VVMTDSEDST VSIQIKLENE GSDEDIETDV LYSPQMALKL 420
ALTEWLQEFG VPHQYSSRQV AHSGAKTSVV DGTPLVAAPS LNATTVVTTV YQEPIMSQGA 480
ALSGEPSVLT KEEEKKQPDE EPMDMVVEKQ EEAEHKNDNQ ILTEIVEAKM AEELRPMDTD 540
KESMAESKSP EMSMQEDCIP DVAPMQTDEQ TQKEQFVPGP NEKPLYTAEP VTLEDLQLLA 600
DLFYLPYEHG PKGAQMLREF QWLRANSSVV SVNCKGKDSE KIEEWRSRAA KFEEMCALVM 660
GMFTRLSNCA NRTILYDMYS YVWDIKSIMS MVKSFVQWLG CRSHSSAQFL IGDQEPWAFR 720
GGLAGEFQRL LPIDGANDLF FQPPPLTPTS KVYTIRPYFP KDEASVYKIC REMYDDGVGF 780
PFQSQPDLIG DKLVGGLLSL SLDYCFVLED EDGICGYALG TVDVTPFIKK CKISWIPFMQ 840
EKYTKPNGDK ELSEAEKIML SFHEEQEVLP ETFLANFPSL IKMDIHKKVT DPSVAKSMMA 900
CLLSSLKANG SRGAFCEVRP DDKRILEFYS KLGCFEIAKM EGFPKDVVIL GRSL 954 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
 GO:0007568; P:aging; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:Compara.
 GO:0070265; P:necrotic cell death; IEA:Compara.
 GO:0010616; P:negative regulation of cardiac muscle adaptation; IEA:Compara.
 GO:0060051; P:negative regulation of protein glycosylation; IEA:Compara.
 GO:0010524; P:positive regulation of calcium ion transport into cytosol; IEA:Compara.
 GO:0031343; P:positive regulation of cell killing; IEA:Compara.
 GO:0051054; P:positive regulation of DNA metabolic process; IEA:Compara.
 GO:0046326; P:positive regulation of glucose import; IEA:Compara.
 GO:0060124; P:positive regulation of growth hormone secretion; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0051901; P:positive regulation of mitochondrial depolarization; IEA:Compara.
 GO:0043243; P:positive regulation of protein complex disassembly; IEA:Compara.
 GO:0045862; P:positive regulation of proteolysis; IEA:Compara.
 GO:0006612; P:protein targeting to membrane; IEA:Compara.
 GO:0048545; P:response to steroid hormone stimulus; IEA:Compara. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR011496; Beta-N-acetylglucosaminidase.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF07555; NAGidase 
SMART
  
PROSITE
  
PRINTS