CPLM-009512

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-009512

UniProt Accession

ISPE_ECOLI; P62615; P24209

Genbank Protein ID

D10264; M77237; U18555; AJ249325; AF179284; AF216300; AB037116; U00096; AP009048

Genbank Nucleotide ID

BAA01106.1; AAA24434.1; AAC43434.1; CAB64963.1; AAF13867.1; AAF29530.1; BAA94247.1; AAC74292.1; BAA36066.1

Protein Name

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Protein Synonyms/Alias

CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene Name

ispE

Gene Synonyms/Alias

ipk; ychB; b1208; JW1199

Created Date

July 27, 2013

Organism

Escherichia coli (strain K12)

NCBI Taxa ID

83333

Lysine Modification

Position	Peptide	Type	References
186	IPTPVIFKDPELPRN	acetylation	[1]
271	WLNGFVAKGANLSPL	acetylation	[1]

Reference

[1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]

Functional Description

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Phosphorylates isopentenyl phosphate at low rates. Also acts on isopentenol, and, much less efficiently, dimethylallyl alcohol. Dimethylallyl monophosphate does not serve as a substrate.

Sequence Annotation

NP_BIND 99 109 ATP (Potential).
ACT_SITE 10 10 By similarity.
ACT_SITE 141 141 By similarity.

Keyword

3D-structure; ATP-binding; Complete proteome; Isoprene biosynthesis; Kinase; Nucleotide-binding; Reference proteome; Transferase.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

283 AA

Protein Sequence

MRTQWPSPAK LNLFLYITGQ RADGYHTLQT LFQFLDYGDT ISIELRDDGD IRLLTPVEGV 60
EHEDNLIVRA ARLLMKTAAD SGRLPTGSGA NISIDKRLPM GGGLGGGSSN AATVLVALNH 120
LWQCGLSMDE LAEMGLTLGA DVPVFVRGHA AFAEGVGEIL TPVDPPEKWY LVAHPGVSIP 180
TPVIFKDPEL PRNTPKRSIE TLLKCEFSND CEVIARKRFR EVDAVLSWLL EYAPSRLTGT 240
GACVFAEFDT ESEARQVLEQ APEWLNGFVA KGANLSPLHR AML 283

Gene Ontology

GO:0050515; F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity; IDA:EcoCyc.
GO:0005524; F:ATP binding; IEA:HAMAP.
GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
GO:0006744; P:ubiquinone biosynthetic process; IDA:EcoCyc.

Interpro

IPR013750; GHMP_kinase_C_dom.
IPR006204; GHMP_kinase_N_dom.
IPR004424; IspE.
IPR020568; Ribosomal_S5_D2-typ_fold.
IPR014721; Ribosomal_S5_D2-typ_fold_subgr.

Pfam

PF08544; GHMP_kinases_C
PF00288; GHMP_kinases_N

SMART

PROSITE

PRINTS