CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013938
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-4 
Protein Synonyms/Alias
 Myosin heavy chain 2b; MyHC-2b; Myosin heavy chain 4 
Gene Name
 Myh4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
22PYLRKSEKERIEAQNubiquitination[1]
30ERIEAQNKPFDAKSSubiquitination[1]
35QNKPFDAKSSVFVVDubiquitination[1]
44SVFVVDAKESYVKATubiquitination[1]
49DAKESYVKATVQSREubiquitination[1]
63EGGKVTAKTEGGATVubiquitination[1]
73GGATVTVKDDQVFSMubiquitination[1]
84VFSMNPPKYDKIEDMubiquitination[1]
185TGESGAGKTVNTKRVubiquitination[1]
237LEAFGNAKTVRNDNSubiquitination[1]
273IETYLLEKSRVTFQLubiquitination[1]
281SRVTFQLKAERSYHIubiquitination[1]
354DEKVAIYKLTGAVMHubiquitination[1]
366VMHYGNMKFKQKQREubiquitination[1]
370GNMKFKQKQREEQAEubiquitination[1]
386DGTEVADKAAYLTSLubiquitination[1]
400LNSADLLKALCYPRVubiquitination[1]
408ALCYPRVKVGNEYVTubiquitination[1]
416VGNEYVTKGQTVQQVubiquitination[1]
453INQQLDTKQPRQYFIubiquitination[1]
505VLEQEEYKKEGIDWEubiquitination[1]
562LYEQHLGKSNNFQKPubiquitination[1]
568GKSNNFQKPKPAKGKubiquitination[1]
570SNNFQKPKPAKGKAEubiquitination[1]
573FQKPKPAKGKAEAHFubiquitination[1]
601IGWLDKNKDPLNETVubiquitination[1]
614TVVGLYQKSGLKTLAubiquitination[1]
618LYQKSGLKTLAFLFSubiquitination[1]
638EAEGGGGKKGGKKKGubiquitination[1]
644GKKGGKKKGSSFQTVubiquitination[1]
661LFRENLNKLMTNLKSubiquitination[1]
667NKLMTNLKSTHPHFVubiquitination[1]
683CLIPNETKTPGAMEHubiquitination[1]
723RILYADFKQRYKVLNubiquitination[1]
727ADFKQRYKVLNASAIubiquitination[1]
743EGQFIDSKKASEKLLubiquitination[1]
748DSKKASEKLLGSIDIubiquitination[1]
761DIDHTQYKFGHTKVFubiquitination[1]
766QYKFGHTKVFFKAGLubiquitination[1]
784LEEMRDEKLAQLITRubiquitination[1]
839PWMKLYFKIKPLLKSubiquitination[1]
841MKLYFKIKPLLKSAEubiquitination[1]
845FKIKPLLKSAETEKEubiquitination[1]
851LKSAETEKEMANMKEubiquitination[1]
857EKEMANMKEDFEKAKubiquitination[1]
862NMKEDFEKAKEDLAKubiquitination[1]
869KAKEDLAKSEAKRKEubiquitination[1]
880KRKELEEKMVALMQEubiquitination[1]
914ERCDQLIKTKIQLEAubiquitination[1]
916CDQLIKTKIQLEAKIubiquitination[1]
922TKIQLEAKIKELTERubiquitination[1]
943INAELTAKKRKLEDEubiquitination[1]
946ELTAKKRKLEDECSEubiquitination[1]
956DECSELKKDIDDLELubiquitination[1]
967DLELTLAKVEKEKHAubiquitination[1]
970LTLAKVEKEKHATENubiquitination[1]
980HATENKVKNLTEEMAubiquitination[1]
995GLDENIAKLTKEKKAubiquitination[1]
998ENIAKLTKEKKALQEubiquitination[1]
1001AKLTKEKKALQEAHQubiquitination[1]
1020DLQAEEDKVNTLTKAubiquitination[1]
1026DKVNTLTKAKTKLEQubiquitination[1]
1028VNTLTKAKTKLEQQVubiquitination[1]
1030TLTKAKTKLEQQVDDubiquitination[1]
1046EGSLEQEKKLRMDLEubiquitination[1]
1077TMDIENDKQQLDEKLubiquitination[1]
1083DKQQLDEKLKKKEFEubiquitination[1]
1097EMSNLQSKIEDEQALubiquitination[1]
1110ALGMQLQKKIKELQAubiquitination[1]
1169SAQIEMNKKREAEFQubiquitination[1]
1170AQIEMNKKREAEFQKubiquitination[1]
1177KREAEFQKMRRDLEEubiquitination[1]
1198ATAAALRKKHADSVAubiquitination[1]
1199TAAALRKKHADSVAEubiquitination[1]
1225KQKLEKEKSELKMEIubiquitination[1]
1229EKEKSELKMEIDDLAubiquitination[1]
1244SNMETVSKAKGNLEKubiquitination[1]
1251KAKGNLEKMCRTLEDubiquitination[1]
1264EDQLSEVKTKEEEQQubiquitination[1]
1266QLSEVKTKEEEQQRLubiquitination[1]
1281INELSTQKARLHTESubiquitination[1]
1298FSRQLDEKDAMVSQLubiquitination[1]
1309VSQLSRGKQAFTQQIubiquitination[1]
1320TQQIEELKRQLEEESubiquitination[1]
1330LEEESKAKNALAHALubiquitination[1]
1358YEEEQEAKAELQRAMubiquitination[1]
1367ELQRAMSKANSEVAQubiquitination[1]
1378EVAQWRTKYETDAIQubiquitination[1]
1394TEELEEAKKKLAQRLubiquitination[1]
1395EELEEAKKKLAQRLQubiquitination[1]
1414HVEAVNSKCASLEKTubiquitination[1]
1420SKCASLEKTKQRLQNubiquitination[1]
1448AACAALDKKQRNFDKubiquitination[1]
1449ACAALDKKQRNFDKVubiquitination[1]
1455KKQRNFDKVLAEWKQubiquitination[1]
1461DKVLAEWKQKYEETQubiquitination[1]
1463VLAEWKQKYEETQAEacetylation[2]
1463VLAEWKQKYEETQAEubiquitination[1]
1476AELEASQKESRSLSTubiquitination[1]
1487SLSTELFKVKNAYEEubiquitination[1]
1489STELFKVKNAYEESLubiquitination[1]
1503LDQLETLKRENKNLQubiquitination[1]
1507ETLKRENKNLQQEISubiquitination[1]
1525EQIAEGGKHIHELEKubiquitination[1]
1532KHIHELEKIKKQIDQubiquitination[1]
1535HELEKIKKQIDQEKSubiquitination[1]
1541KKQIDQEKSELQASLubiquitination[1]
1561SLEHEEGKILRIQLEacetylation[2]
1561SLEHEEGKILRIQLEubiquitination[1]
1573QLELNQVKSEIDRKIubiquitination[1]
1579VKSEIDRKIAEKDEEubiquitination[1]
1583IDRKIAEKDEEIDQLubiquitination[1]
1591DEEIDQLKRNHLRVVubiquitination[1]
1620NDALRIKKKMEGDLNubiquitination[1]
1621DALRIKKKMEGDLNEubiquitination[1]
1655RNTQGMLKDTQLHLDubiquitination[1]
1672LRGQDDLKEQLAMVEubiquitination[1]
1731NTSLINTKKKLETDIubiquitination[1]
1733SLINTKKKLETDISQubiquitination[1]
1761NAEEKAKKAITDAAMubiquitination[1]
1774AMMAEELKKEQDTSAubiquitination[1]
1775MMAEELKKEQDTSAHubiquitination[1]
1788AHLERMKKNMEQTVKubiquitination[1]
1795KNMEQTVKDLQHRLDubiquitination[1]
1810EAEQLALKGGKKQIQubiquitination[1]
1813QLALKGGKKQIQKLEubiquitination[1]
1814LALKGGKKQIQKLEAubiquitination[1]
1835NEVENEQKRNIEAVKubiquitination[1]
1842KRNIEAVKGLRKHERubiquitination[1]
1852RKHERRVKELTYQTEubiquitination[1]
1863YQTEEDRKNVLRLQDubiquitination[1]
1874RLQDLVDKLQTKVKAacetylation[2]
1874RLQDLVDKLQTKVKAubiquitination[1]
1878LVDKLQTKVKAYKRQubiquitination[1]
1899QSNVNLAKFRKIQHEubiquitination[1]
1923IAESQVNKLRVKSREubiquitination[1]
1934KSREVHTKVISEE**ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 
Functional Description
 Muscle contraction. 
Sequence Annotation
 DOMAIN 1 784 Myosin head-like.
 DOMAIN 785 814 IQ.
 NP_BIND 179 186 ATP (Potential).
 REGION 659 681 Actin-binding (By similarity).
 REGION 761 775 Actin-binding (By similarity).
 MOD_RES 130 130 N6,N6,N6-trimethyllysine (Potential).
 MOD_RES 952 952 Phosphoserine (By similarity).
 MOD_RES 1203 1203 Phosphoserine (By similarity).
 MOD_RES 1928 1928 Phosphoserine (By similarity).
 MOD_RES 1933 1933 Phosphothreonine (By similarity).
 CROSSLNK 1813 1813 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Thick filament; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1939 AA 
Protein Sequence
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESYVKA TVQSREGGKV 60
TAKTEGGATV TVKDDQVFSM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 120
LFCVTVNPYK WLPVYNPEVV AAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 180
SGAGKTVNTK RVIQYFATIA VTGDKKKEEA TSGKMQGTLE DQIISANPLL EAFGNAKTVR 240
NDNSSRFGKF IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPEL 300
IEMLLITTNP YDFAYVSQGE ITVPSIDDQE ELMATDTAVD ILGFSADEKV AIYKLTGAVM 360
HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK ALCYPRVKVG NEYVTKGQTV 420
QQVYNSVGAL AKSMYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNTLEQLC 480
INFTNEKLQQ FFNHHMFVLE QEEYKKEGID WEFIDFGMDL AACIELIEKP MGIFSILEEE 540
CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNIIGWLDKN 600
KDPLNETVVG LYQKSGLKTL AFLFSGGQAA EAEGGGGKKG GKKKGSSFQT VSALFRENLN 660
KLMTNLKSTH PHFVRCLIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA 720
DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIDIDHTQY KFGHTKVFFK AGLLGTLEEM 780
RDEKLAQLIT RTQAVCRGYL MRVEFKKMME RRESIFCIQY NVRAFMNVKH WPWMKLYFKI 840
KPLLKSAETE KEMANMKEDF EKAKEDLAKS EAKRKELEEK MVALMQEKND LQLQVQAEAD 900
GLADAEERCD QLIKTKIQLE AKIKELTERA EDEEEINAEL TAKKRKLEDE CSELKKDIDD 960
LELTLAKVEK EKHATENKVK NLTEEMAGLD ENIAKLTKEK KALQEAHQQT LDDLQAEEDK 1020
VNTLTKAKTK LEQQVDDLEG SLEQEKKLRM DLERAKRKLE GDLKLAQEST MDIENDKQQL 1080
DEKLKKKEFE MSNLQSKIED EQALGMQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR 1140
SDLSRELEEI SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEEATLQHE ATAAALRKKH 1200
ADSVAELGEQ IDNLQRVKQK LEKEKSELKM EIDDLASNME TVSKAKGNLE KMCRTLEDQL 1260
SEVKTKEEEQ QRLINELSTQ KARLHTESGE FSRQLDEKDA MVSQLSRGKQ AFTQQIEELK 1320
RQLEEESKAK NALAHALQSA RHDCDLLREQ YEEEQEAKAE LQRAMSKANS EVAQWRTKYE 1380
TDAIQRTEEL EEAKKKLAQR LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN 1440
AACAALDKKQ RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDQLE 1500
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKIKKQIDQE KSELQASLEE AEASLEHEEG 1560
KILRIQLELN QVKSEIDRKI AEKDEEIDQL KRNHLRVVES MQSTLDAEIR SRNDALRIKK 1620
KMEGDLNEME IQLNHANRQA AEAIRNLRNT QGMLKDTQLH LDDALRGQDD LKEQLAMVER 1680
RANLMQAEIE ELRASLEQTE RSRRVAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ 1740
IQGEMEDIVQ EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQHR 1800
LDEAEQLALK GGKKQIQKLE ARVRELENEV ENEQKRNIEA VKGLRKHERR VKELTYQTEE 1860
DRKNVLRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF RKIQHELEEA EERADIAESQ 1920
VNKLRVKSRE VHTKVISEE 1939 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0030016; C:myofibril; IEA:UniProtKB-SubCell.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003774; F:motor activity; IEA:InterPro.
 GO:0006936; P:muscle contraction; IEA:Compara.
 GO:0014823; P:response to activity; IDA:MGI. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR015650; Myosin_1/23/4/7/8/13/15.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.