CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008346
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Annexin A11 
Protein Synonyms/Alias
 56 kDa autoantigen; Annexin XI; Annexin-11; Calcyclin-associated annexin 50; CAP-50 
Gene Name
 ANXA11 
Gene Synonyms/Alias
 ANX11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
214EVLRKAMKGFGTDEQubiquitination[1, 2]
248SFKTAYGKDLIKDLKacetylation[3]
248SFKTAYGKDLIKDLKubiquitination[2]
252AYGKDLIKDLKSELSubiquitination[2]
255KDLIKDLKSELSGNFacetylation[3]
255KDLIKDLKSELSGNFubiquitination[2]
264ELSGNFEKTILALMKubiquitination[2, 4, 5]
271KTILALMKTPVLFDIubiquitination[2, 6]
282LFDIYEIKEAIKGVGubiquitination[2, 6]
286YEIKEAIKGVGTDEAubiquitination[2]
320AYKAEFKKTLEEAIRubiquitination[2]
378RLGTDESKFNAVLCSubiquitination[1, 2]
408MTGRDIEKSICREMSubiquitination[2]
427EGMLAVVKCLKNTPAubiquitination[2]
430LAVVKCLKNTPAFFAubiquitination[2]
479EYKRMYGKSLYHDISacetylation[3]
479EYKRMYGKSLYHDISubiquitination[2]
499DYRKILLKICGGND*ubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis. 
Sequence Annotation
 REPEAT 209 269 Annexin 1.
 REPEAT 281 341 Annexin 2.
 REPEAT 365 425 Annexin 3.
 REPEAT 440 500 Annexin 4.
 MOD_RES 248 248 N6-acetyllysine.
 MOD_RES 255 255 N6-acetyllysine.
 MOD_RES 479 479 N6-acetyllysine.  
Keyword
 Acetylation; Annexin; Calcium; Calcium/phospholipid-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MSYPGYPPPP GGYPPAAPGG GPWGGAAYPP PPSMPPIGLD NVATYAGQFN QDYLSGMAAN 60
MSGTFGGANM PNLYPGAPGA GYPPVPPGGF GQPPSAQQPV PPYGMYPPPG GNPPSRMPSY 120
PPYPGAPVPG QPMPPPGQQP PGAYPGQPPV TYPGQPPVPL PGQQQPVPSY PGYPGSGTVT 180
PAVPPTQFGS RGTITDAPGF DPLRDAEVLR KAMKGFGTDE QAIIDCLGSR SNKQRQQILL 240
SFKTAYGKDL IKDLKSELSG NFEKTILALM KTPVLFDIYE IKEAIKGVGT DEACLIEILA 300
SRSNEHIREL NRAYKAEFKK TLEEAIRSDT SGHFQRLLIS LSQGNRDEST NVDMSLAQRD 360
AQELYAAGEN RLGTDESKFN AVLCSRSRAH LVAVFNEYQR MTGRDIEKSI CREMSGDLEE 420
GMLAVVKCLK NTPAFFAERL NKAMRGAGTK DRTLIRIMVS RSETDLLDIR SEYKRMYGKS 480
LYHDISGDTS GDYRKILLKI CGGND 505 
Gene Ontology
 GO:0042582; C:azurophil granule; IDA:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0045335; C:phagocytic vesicle; IDA:UniProtKB.
 GO:0042581; C:specific granule; IDA:UniProtKB.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; IEA:Compara.
 GO:0005544; F:calcium-dependent phospholipid binding; IEA:UniProtKB-KW.
 GO:0008429; F:phosphatidylethanolamine binding; IEA:Compara.
 GO:0007109; P:cytokinesis, completion of separation; IMP:UniProtKB.
 GO:0006909; P:phagocytosis; IEP:UniProtKB.
 GO:0051592; P:response to calcium ion; IDA:UniProtKB. 
Interpro
 IPR001464; Annexin.
 IPR018502; Annexin_repeat.
 IPR018252; Annexin_repeat_CS.
 IPR008157; AnnexinXI. 
Pfam
 PF00191; Annexin 
SMART
 SM00335; ANX 
PROSITE
 PS00223; ANNEXIN 
PRINTS
 PR00196; ANNEXIN.
 PR01810; ANNEXINXI.