CPLM-022636

Genbank Nucleotide ID

Calcium-binding mitochondrial carrier protein Aralar2

Protein Synonyms/Alias

Citrin; Mitochondrial aspartate glutamate carrier 2; Solute carrier family 25 member 13

Mus musculus (Mouse)

Position	Peptide	Type	References
18	RADPAELKAIFLKYA	acetylation	[1, 2]
18	RADPAELKAIFLKYA	ubiquitination	[3]
29	LKYASIEKNGEFFMS	ubiquitination	[3]
104	QLFDKAGKGEVTFED	acetylation	[4]
177	RDNAKTGKVSAIDFR	acetylation	[5]
177	RDNAKTGKVSAIDFR	phosphoglycerylation	[6]
235	NNMELIRKIYSTLAG	acetylation	[2]
245	STLAGNRKDVEVTKE	acetylation	[7]
251	RKDVEVTKEEFALAA	acetylation	[7]
251	RKDVEVTKEEFALAA	ubiquitination	[3]
354	VYPIDLVKTRMQNQR	acetylation	[2]
354	VYPIDLVKTRMQNQR	ubiquitination	[3]
373	FVGELMYKNSFDCFK	acetylation	[2, 5, 7]
380	KNSFDCFKKVLRYEG	acetylation	[7]
406	LLGVAPEKAIKLTVN	acetylation	[2, 5]
406	LLGVAPEKAIKLTVN	ubiquitination	[3]
409	VAPEKAIKLTVNDFV	ubiquitination	[3]
485	LGFFGIYKGAKACFL	acetylation	[1, 2, 5, 7, 8, 9]
485	LGFFGIYKGAKACFL	succinylation	[9]
485	LGFFGIYKGAKACFL	ubiquitination	[3]
546	VTPADVIKTRLQVAA	acetylation	[2]
577	ILREEGPKALWKGVA	acetylation	[7]
581	EGPKALWKGVAARVF	acetylation	[5, 7, 9]
581	EGPKALWKGVAARVF	succinylation	[9]
642	PDHVGGYKLAVATFA	acetylation	[5]
663	GLYLPLFKPSASTSK	acetylation	[2]

[1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[4] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[6] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
Moellering RE, Cravatt BF.
Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
[7] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[9] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]

Functional Description

Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.

DOMAIN 51 86 EF-hand 1.
DOMAIN 87 122 EF-hand 2.
DOMAIN 123 157 EF-hand 3.
DOMAIN 158 193 EF-hand 4.
REPEAT 327 419 Solcar 1.
REPEAT 427 511 Solcar 2.
REPEAT 519 607 Solcar 3.
MOD_RES 18 18 N6-acetyllysine.
MOD_RES 485 485 N6-acetyllysine.

Acetylation; Calcium; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
GO:0005509; F:calcium ion binding; IEA:Compara.
GO:0015183; F:L-aspartate transmembrane transporter activity; ISS:UniProtKB.
GO:0005313; F:L-glutamate transmembrane transporter activity; IMP:MGI.
GO:0006754; P:ATP biosynthetic process; IEA:Compara.
GO:0043490; P:malate-aspartate shuttle; IMP:MGI.
GO:0051592; P:response to calcium ion; ISS:UniProtKB.

IPR011992; EF-hand-like_dom.
IPR002048; EF_hand_dom.
IPR002067; Mit_carrier.
IPR018108; Mitochondrial_sb/sol_carrier.
IPR023395; Mt_carrier_dom.

PF13405; EF_hand_4
PF00153; Mito_carr

PS00018; EF_HAND_1
PS50222; EF_HAND_2
PS50920; SOLCAR

PR00926; MITOCARRIER.