CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032004
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multidrug resistance-associated protein 4 
Protein Synonyms/Alias
 cDNA FLJ52311, highly similar to Multidrug resistance-associated protein 4 
Gene Name
 ABCC4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66ELQGFWDKEVLRAENubiquitination[1, 2, 3]
77RAENDAQKPSLTRAIubiquitination[1, 2, 3]
189LFSSLRSKTATFTDAubiquitination[1, 2, 4]
218IKMYAWEKSFSNLITubiquitination[1, 4]
229NLITNLRKKEISKILubiquitination[1]
234LRKKEISKILRSSCLubiquitination[2]
440IKACALKKDLQLLEDubiquitination[1, 3, 4]
465TTLSGGQKARVNLARubiquitination[1]
532ASQILILKDGKMVQKubiquitination[1]
539KDGKMVQKGTYTEFLubiquitination[1]
547GTYTEFLKSGIDFGSubiquitination[1, 3, 4]
557IDFGSLLKKDNEESEubiquitination[1, 3, 4]
558DFGSLLKKDNEESEQubiquitination[1, 3]
595QSSRPSLKDGALESQubiquitination[1, 3]
620EENRSEGKVGFQAYKubiquitination[1]
627KVGFQAYKNYFRAGAubiquitination[4, 5]
732KMFESILKAPVLFFDubiquitination[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 784 AA 
Protein Sequence
MLPVYQEVKP NPLQDANLCS RVFFWWLNPL FKIGHKRRLE EDDMYSVLPE DRSQHLGEEL 60
QGFWDKEVLR AENDAQKPSL TRAIIKCYWK SYLVLGIFTL IEALRLSNMA MGKTTTGQIV 120
NLLSNDVNKF DQVTVFLHFL WAGPLQAIAV TALLWMEIGI SCLAGMAVLI ILLPLQSCFG 180
KLFSSLRSKT ATFTDARIRT MNEVITGIRI IKMYAWEKSF SNLITNLRKK EISKILRSSC 240
LRGMNLASFF SASKIIVFVT FTTYVLLGSV ITASRVFVAV TLYGAVRLTV TLFFPSAIER 300
VSEAIVSIRR IQTFLLLDEI SQRNRQLPSD GKKMVHVQDF TAFWDKASET PTLQGLSFTV 360
RPGELLAVVG PVGAGKSSLL SAVLGELAPS HGLVSVHGRI AYVSQQPWVF SGTLRSNILF 420
GKKYEKERYE KVIKACALKK DLQLLEDGDL TVIGDRGTTL SGGQKARVNL ARAVYQDADI 480
YLLDDPLSAV DAEVSRHLFE LCICQILHEK ITILVTHQLQ YLKAASQILI LKDGKMVQKG 540
TYTEFLKSGI DFGSLLKKDN EESEQPPVPG TPTLRNRTFS ESSVWSQQSS RPSLKDGALE 600
SQDTENVPVT LSEENRSEGK VGFQAYKNYF RAGAHWIVFI FLILLNTAAQ VAYVLQDWWL 660
SYWANKQSML NVTVNGGGNV TEKLDLNWYL GIYSGLTVAT VLFGIARSLL VFYVLVNSSQ 720
TLHNKMFESI LKAPVLFFDR NPIGRILNRF SKDIGHLDDL LPLTFLDFIQ RWDLAVLSWL 780
VSNS 784 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042626; F:ATPase activity, coupled to transmembrane movement of substances; IEA:InterPro.
 GO:0006200; P:ATP catabolic process; IEA:GOC. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS