CPLM-041836

Genbank Nucleotide ID

Histone H2B type 2-B

Protein Synonyms/Alias

MCG48964, isoform CRA_a

Mus musculus (Mouse)

Position	Peptide	Type	References
6	**MPDPAKSAPAPKK	acetylation	[1, 2, 3]
12	AKSAPAPKKGSKKAV	acetylation	[2, 3, 4, 5, 6, 7]
13	KSAPAPKKGSKKAVT	acetylation	[2, 4, 5, 6]
16	PAPKKGSKKAVTKVQ	acetylation	[2, 3, 4, 5]
17	APKKGSKKAVTKVQK	acetylation	[1, 2, 4, 5, 6, 8]
21	GSKKAVTKVQKKDGK	acetylation	[1, 2, 3, 4, 5, 6, 8]
24	KAVTKVQKKDGKKRK	acetylation	[1, 2]
35	KKRKRSRKESYSVYV	acetylation	[2, 5]
35	KKRKRSRKESYSVYV	succinylation	[2]
35	KKRKRSRKESYSVYV	ubiquitination	[9]
44	SYSVYVYKVLKQVHP	acetylation	[2, 8]
44	SYSVYVYKVLKQVHP	succinylation	[2]
44	SYSVYVYKVLKQVHP	ubiquitination	[9]
47	VYVYKVLKQVHPDTG	acetylation	[2, 4, 5, 8]
47	VYVYKVLKQVHPDTG	succinylation	[2]
47	VYVYKVLKQVHPDTG	ubiquitination	[9]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
[8] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[9] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).

Chromosome; Complete proteome; DNA-binding; Nucleosome core; Nucleus; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
GO:0005634; C:nucleus; IEA:UniProtKB-KW.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0006334; P:nucleosome assembly; IEA:InterPro.

IPR009072; Histone-fold.
IPR007125; Histone_core_D.
IPR000558; Histone_H2B.

PR00621; HISTONEH2B.