CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032988
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nucleolin 
Protein Synonyms/Alias
  
Gene Name
 NCL 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54KVVVSPTKKVAVATPacetylation[1]
63VAVATPAKKAAVTPGacetylation[1]
64AVATPAKKAAVTPGKubiquitination[2]
71KAAVTPGKKAAATPAacetylation[1]
86KKTVTPAKAVTTPGKacetylation[3, 4]
86KKTVTPAKAVTTPGKubiquitination[1, 2]
93KAVTTPGKKGATPGKacetylation[1, 5]
94AVTTPGKKGATPGKAacetylation[5]
100KKGATPGKALVATPGacetylation[1, 3, 4]
100KKGATPGKALVATPGubiquitination[6]
108ALVATPGKKGAAIPAacetylation[1, 4]
109LVATPGKKGAAIPAKubiquitination[2]
116KGAAIPAKGAKNGKNacetylation[4]
119AIPAKGAKNGKNAKKacetylation[4]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Identification of lysine acetyltransferase p300 substrates using 4-pentynoyl-coenzyme A and bioorthogonal proteomics.
 Yang YY, Grammel M, Hang HC.
 Bioorg Med Chem Lett. 2011 Sep 1;21(17):4976-9. [PMID: 21669532]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 166 AA 
Protein Sequence
MAPPPKEVEE DSEDEEMSED EEDDSSGEEV VIPQKKGKKA AATSAKKVVV SPTKKVAVAT 60
PAKKAAVTPG KKAAATPAKK TVTPAKAVTT PGKKGATPGK ALVATPGKKG AAIPAKGAKN 120
GKNAKKEDSD EEEDDDSEED EEDDEDEDED EDEIEPAAMK AAAAAP 166 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005654; C:nucleoplasm; IEA:Compara. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS