CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018255
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger CCCH domain-containing protein 15 
Protein Synonyms/Alias
 DRG family-regulatory protein 1; Likely ortholog of mouse immediate early response erythropoietin 4 
Gene Name
 ZC3H15 
Gene Synonyms/Alias
 DFRP1; LEREPO4; HSPC303; HT010; MSTP012; PP730 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27KEKIIEDKTFGLKNKubiquitination[1, 2, 3]
44AKQQKFIKAVTHQVKubiquitination[3, 4]
51KAVTHQVKFGQQNPRubiquitination[1, 3]
86QELNELFKPVVAAQKubiquitination[4]
259LESFLAWKKRKRQEKubiquitination[4]
260ESFLAWKKRKRQEKIubiquitination[4]
285KADFKAGKALVISGRubiquitination[5]
362FSTYTSDKDENKLSEacetylation[6]
362FSTYTSDKDENKLSEubiquitination[2, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Protects DRG1 from proteolytic degradation (By similarity). 
Sequence Annotation
 ZN_FING 99 126 C3H1-type 1.
 ZN_FING 174 212 C3H1-type 2.
 REGION 236 260 Required for interaction with DRG1 (By
 MOD_RES 351 351 Phosphoserine.
 MOD_RES 381 381 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 426 AA 
Protein Sequence
MPPKKQAQAG GSKKAEQKKK EKIIEDKTFG LKNKKGAKQQ KFIKAVTHQV KFGQQNPRQV 60
AQSEAEKKLK KDDKKKELQE LNELFKPVVA AQKISKGADP KSVVCAFFKQ GQCTKGDKCK 120
FSHDLTLERK CEKRSVYIDA RDEELEKDTM DNWDEKKLEE VVNKKHGEAE KKKPKTQIVC 180
KHFLEAIENN KYGWFWVCPG GGDICMYRHA LPPGFVLKKD KKKEEKEDEI SLEDLIERER 240
SALGPNVTKI TLESFLAWKK RKRQEKIDKL EQDMERRKAD FKAGKALVIS GREVFEFRPE 300
LVNDDDEEAD DTRYTQGTGG DEVDDSVSVN DIDLSLYIPR DVDETGITVA SLERFSTYTS 360
DKDENKLSEA SGGRAENGER SDLEEDNERE GTENGAIDAV PVDENLFTGE DLDELEEELN 420
TLDLEE 426 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0019221; P:cytokine-mediated signaling pathway; IEA:Compara. 
Interpro
 IPR000571; Znf_CCCH. 
Pfam
 PF00642; zf-CCCH 
SMART
 SM00356; ZnF_C3H1 
PROSITE
 PS50103; ZF_C3H1 
PRINTS