CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000532
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-galactosidase 
Protein Synonyms/Alias
 Melibiase 
Gene Name
 melA 
Gene Synonyms/Alias
 BSU30300 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
177HSVQVCTKDLFKALGacetylation[1]
207MAWLLEVKKDGTDLYacetylation[1]
391EAAVTRKKEAVYQAAacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
  
Sequence Annotation
 NP_BIND 2 68 NAD (By similarity).
 ACT_SITE 170 170 Proton donor (By similarity).
 METAL 169 169 Manganese (By similarity).
 METAL 199 199 Manganese (By similarity).
 BINDING 148 148 Substrate (By similarity).  
Keyword
 Carbohydrate metabolism; Complete proteome; Glycosidase; Hydrolase; Manganese; Metal-binding; NAD; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 432 AA 
Protein Sequence
MKKITFIGAG STIFAKNVLG DCLLTEALNG FEFALYDIDP KRLQESQLML ENLRDRYNPS 60
VAINSYDDRK LALQNAGYVI NAIQVGGYKP STVIDFEIPK RYGLRQTIAD TVGIGGIFRS 120
LRTIPVLFDI AKDMEEMCPD AWFLNYTNPM ATLTGAMLRY TNIKTIGLCH SVQVCTKDLF 180
KALGMEHDGI EERIAGINHM AWLLEVKKDG TDLYPEIKRR AKEKQKTKHH DMVRFELMDK 240
FGYYVTESSE HNAEYHPYFI KRNYPELISE LQIPLDEYPR RCVKQIENWE KMRDDIVNNK 300
NLTHERSKEY GSRIIEAMET NEPFTFGGNV LNTGLITNLP SKAVVEVTCV ADRKKITPCF 360
AGELPEQLAA LNRTNINTQL MTIEAAVTRK KEAVYQAAML DPHTSAELSM KDIISMCDDL 420
FAAHGDWLPE YK 432 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
 GO:0052692; F:raffinose alpha-galactosidase activity; IEA:EC.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR019802; GlycHydrolase_4_CS.
 IPR001088; Glyco_hydro_4.
 IPR022616; Glyco_hydro_4_C.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02056; Glyco_hydro_4
 PF11975; Glyco_hydro_4C 
SMART
  
PROSITE
 PS01324; GLYCOSYL_HYDROL_F4 
PRINTS
 PR00732; GLHYDRLASE4.