CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009072
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Breast cancer anti-estrogen resistance protein 1 
Protein Synonyms/Alias
 CRK-associated substrate; Cas scaffolding protein family member 1; p130cas 
Gene Name
 BCAR1 
Gene Synonyms/Alias
 CAS; CASS1; CRKAS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
702QLELQQLKQFERLEQubiquitination[1, 2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. 
Sequence Annotation
 DOMAIN 3 65 SH3.
 REGION 115 416 Substrate for kinases (By similarity).
 REGION 746 796 Divergent helix-loop-helix motif.
 MOTIF 635 643 SH3-binding (Potential).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 128 128 Phosphotyrosine; by SRC.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 224 224 Phosphotyrosine (By similarity).
 MOD_RES 234 234 Phosphotyrosine (By similarity).
 MOD_RES 249 249 Phosphotyrosine; by ABL1.
 MOD_RES 269 269 Phosphothreonine.
 MOD_RES 292 292 Phosphoserine.
 MOD_RES 327 327 Phosphotyrosine (By similarity).
 MOD_RES 362 362 Phosphotyrosine (By similarity).
 MOD_RES 410 410 Phosphotyrosine (By similarity).
 MOD_RES 428 428 Phosphoserine.
 MOD_RES 437 437 Phosphoserine.
 MOD_RES 639 639 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell adhesion; Cell junction; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain; SH3-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 870 AA 
Protein Sequence
MNHLNVLAKA LYDNVAESPD ELSFRKGDIM TVLEQDTQGL DGWWLCSLHG RQGIVPGNRL 60
KILVGMYDKK PAGPGPGPPA TPAQPQPGLH APAPPASQYT PMLPNTYQPQ PDSVYLVPTP 120
SKAQQGLYQV PGPSPQFQSP PAKQTSTFSK QTPHHPFPSP ATDLYQVPPG PGGPAQDIYQ 180
VPPSAGMGHD IYQVPPSMDT RSWEGTKPPA KVVVPTRVGQ GYVYEAAQPE QDEYDIPRHL 240
LAPGPQDIYD VPPVRGLLPS QYGQEVYDTP PMAVKGPNGR DPLLEVYDVP PSVEKGLPPS 300
NHHAVYDVPP SVSKDVPDGP LLREETYDVP PAFAKAKPFD PARTPLVLAA PPPDSPPAED 360
VYDVPPPAPD LYDVPPGLRR PGPGTLYDVP RERVLPPEVA DGGVVDSGVY AVPPPAEREA 420
PAEGKRLSAS STGSTRSSQS ASSLEVAGPG REPLELEVAV EALARLQQGV SATVAHLLDL 480
AGSAGATGSW RSPSEPQEPL VQDLQAAVAA VQSAVHELLE FARSAVGNAA HTSDRALHAK 540
LSRQLQKMED VHQTLVAHGQ ALDAGRGGSG ATLEDLDRLV ACSRAVPEDA KQLASFLHGN 600
ASLLFRRTKA TAPGPEGGGT LHPNPTDKTS SIQSRPLPSP PKFTSQDSPD GQYENSEGGW 660
MEDYDYVHLQ GKEEFEKTQK ELLEKGSITR QGKSQLELQQ LKQFERLEQE VSRPIDHDLA 720
NWTPAQPLAP GRTGGLGPSD RQLLLFYLEQ CEANLTTLTN AVDAFFTAVA TNQPPKIFVA 780
HSKFVILSAH KLVFIGDTLS RQAKAADVRS QVTHYSNLLC DLLRGIVATT KAAALQYPSP 840
SAAQDMVERV KELGHSTQQF RRVLGQLAAA 870 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; ISS:UniProtKB.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0007015; P:actin filament organization; IDA:UniProtKB.
 GO:0050853; P:B cell receptor signaling pathway; IDA:UniProtKB.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0060326; P:cell chemotaxis; IMP:BHF-UCL.
 GO:0051301; P:cell division; NAS:UniProtKB.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IMP:BHF-UCL.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0007186; P:G-protein coupled receptor signaling pathway; ISS:UniProtKB.
 GO:0048012; P:hepatocyte growth factor receptor signaling pathway; IMP:BHF-UCL.
 GO:0008286; P:insulin receptor signaling pathway; ISS:UniProtKB.
 GO:0007229; P:integrin-mediated signaling pathway; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; ISS:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0010595; P:positive regulation of endothelial cell migration; IDA:BHF-UCL.
 GO:0042981; P:regulation of apoptotic process; TAS:UniProtKB.
 GO:0001558; P:regulation of cell growth; TAS:UniProtKB.
 GO:0050852; P:T cell receptor signaling pathway; NAS:UniProtKB.
 GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IMP:BHF-UCL. 
Interpro
 IPR021901; CAS_DUF3513.
 IPR014928; Serine_rich.
 IPR001452; SH3_domain.
 IPR013315; Spectrin_alpha_SH3. 
Pfam
 PF12026; DUF3513
 PF08824; Serine_rich
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.
 PR01887; SPECTRNALPHA.