UniProt Accession

 HNT1_YEAST; Q04344; D6VRM5 

Genbank Protein ID

 X56956; Z74173; BK006938 

Genbank Nucleotide ID

 CAA40275.1; CAA98693.1; DAA11735.1 

Protein Name

 Hit family protein 1 

Protein Synonyms/Alias

 Adenosine 5'-monophosphoramidase 

Gene Name

 HNT1 

Gene Synonyms/Alias

 HIT1; YDL125C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
32	CIFCKIIKSEIPSFK	acetylation	[1]
39	KSEIPSFKLIETKYS	acetylation	[1]
44	SFKLIETKYSYAFLD	ubiquitination	[2]
70	IPKYHGAKLHDIPDE	ubiquitination	[2]
148	DKLGKLHKELLAKLE	acetylation	[1]
153	LHKELLAKLEGSD**	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Essential for growth on galactose media at elevated temperatures. 

Sequence Annotation

 DOMAIN 26 129 HIT.
 MOTIF 114 118 Histidine triad motif.
 ACT_SITE 116 116 Tele-AMP-histidine intermediate.  

Keyword

 Complete proteome; Hydrolase; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 158 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0016787; F:hydrolase activity; IDA:SGD.
 GO:0000166; F:nucleotide binding; ISS:SGD.
 GO:0009117; P:nucleotide metabolic process; IDA:SGD. 

Interpro

 IPR019808; Histidine_triad_CS.
 IPR001310; Histidine_triad_HIT.
 IPR011146; HIT-like. 

Pfam

 PF01230; HIT 

SMART

  

PROSITE

 PS00892; HIT_1
 PS51084; HIT_2 

PRINTS

 PR00332; HISTRIAD.