CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008106
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Asparagine synthetase [glutamine-hydrolyzing] 
Protein Synonyms/Alias
 Glutamine-dependent asparagine synthetase 
Gene Name
 Asns 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
395EESERLLKELYLFDVacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 DOMAIN 2 191 Glutamine amidotransferase type-2.
 DOMAIN 213 536 Asparagine synthetase.
 NP_BIND 363 364 ATP (By similarity).
 REGION 49 53 Glutamine binding (By similarity).
 REGION 75 77 Glutamine binding (By similarity).
 ACT_SITE 2 2 For GATase activity (By similarity).
 BINDING 97 97 Glutamine (By similarity).
 BINDING 256 256 ATP; via carbonyl oxygen (By similarity).
 BINDING 288 288 ATP; via amide nitrogen and carbonyl
 MOD_RES 385 385 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 561 AA 
Protein Sequence
MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL AVVDPLFGMQ 60
PIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI ILHLYDKGGI EKTICMLDGV 120
FAFILLDTAN KKVFLGRDTY GVRPLFKALT EDGFLAVCSE AKGLVSLKHS TTPFLKVEPF 180
LPGHYEVLDL KPNGKVASVE MVKYHHCTDE PLHAIYDSVE KLFPGFEIET VKNNLRILFN 240
NAIKKRLMTD RRIGCLLSGG LDSSLVAASL LKQLKEAQVP YALQTFAIGM EDSPDLLAAR 300
KVANYIGSEH HEVLFNSEEG IQSLDEVIFS LETYDITTVR ASVGMYLISK YIRKNTDSVV 360
IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF DVLRADRTTA AHGLELRVPF 420
LDHRFSSYYL SLPPEMRIPK DGIEKHLLRE TFEDSNLLPK EILWRPKEAF SDGITSVKNS 480
WFKILQDFVE HQVDDAMMSE ASQKFPFNTP QTKEGYYYRQ IFEHHYPGRA DWLTHYWMPK 540
WINATDPSAR TLTHYKSTAK A 561 
Gene Ontology
 GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0048037; F:cofactor binding; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0006529; P:asparagine biosynthetic process; IDA:RGD.
 GO:0042149; P:cellular response to glucose starvation; IEP:RGD.
 GO:0032870; P:cellular response to hormone stimulus; IEP:RGD.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0070981; P:L-asparagine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0045931; P:positive regulation of mitotic cell cycle; IEA:Compara.
 GO:0043200; P:response to amino acid stimulus; IEP:RGD.
 GO:0032354; P:response to follicle-stimulating hormone stimulus; IEP:RGD.
 GO:0009416; P:response to light stimulus; IEP:RGD.
 GO:0009612; P:response to mechanical stimulus; IEP:RGD.
 GO:0031427; P:response to methotrexate; IEP:RGD.
 GO:0009636; P:response to toxic substance; IEP:RGD. 
Interpro
 IPR006426; Asn_synth_AEB.
 IPR001962; Asn_synthase.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00733; Asn_synthase
 PF13537; GATase_7 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2 
PRINTS