CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear body protein SP140-like protein 
Protein Synonyms/Alias
  
Gene Name
 SP140L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87DRELITNKMFEDSEDubiquitination[1]
145IHIYKSFKNAIQDKLubiquitination[1]
151FKNAIQDKLSFQESDubiquitination[1]
160SFQESDRKEREERPDubiquitination[1, 2]
169REERPDIKLSLKQGEubiquitination[1]
173PDIKLSLKQGEVPESubiquitination[1, 2]
207ANNSTLGKPKRKRRKubiquitination[1, 2, 3]
209NSTLGKPKRKRRKKKubiquitination[1]
229RMGTRTQKNNQQNDNubiquitination[1]
238NQQNDNSKADGQLVSubiquitination[1, 2]
248GQLVSSEKKANMNLKubiquitination[1, 2]
249QLVSSEKKANMNLKDubiquitination[1]
255KKANMNLKDLSKIRGubiquitination[1, 4]
290VRSRASRKHKDETVDubiquitination[1]
318KGILHKEKLEQGTLAubiquitination[1, 5]
389RNKKRILKSQNNSSVubiquitination[1]
449NCIFCRMKESPGSQQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 33 149 HSR.
 DOMAIN 293 374 SAND.
 DOMAIN 484 554 Bromo.
 ZN_FING 403 449 PHD-type.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 580 AA 
Protein Sequence
MAGGGSDLST RGLNGGVSQV ANEMNHLPAH SQSLQRLFTE DQDVDEGLVY DTVFKHFKRH 60
KLEISNAIKK TFPFLEGLRD RELITNKMFE DSEDSCRNLV PVQRVVYNVL SELEKTFNLS 120
VLEALFSEVN MQEYPDLIHI YKSFKNAIQD KLSFQESDRK EREERPDIKL SLKQGEVPES 180
PEARKESDQA CGKMDTVDIA NNSTLGKPKR KRRKKKGHGW SRMGTRTQKN NQQNDNSKAD 240
GQLVSSEKKA NMNLKDLSKI RGRKRGKPGT HFTQSDRAPQ KRVRSRASRK HKDETVDFQA 300
PLLPVTCGGV KGILHKEKLE QGTLAKCIQT EDGKWFTPME FEIKGGYARS KNWRLSVRCG 360
GWPLRRLMEE GSLPNPPRIY YRNKKRILKS QNNSSVDPCM RNLDECEVCR DGGELFCCDT 420
CSRVFHEDCH IPPVESEKTP WNCIFCRMKE SPGSQQCCQE SEVLERQMCP EEQLKCEFLL 480
LKVYCCSESS FFAKIPYYYY IREACQGLKE PMWLDKIKKR LNEHGYPQVE GFVQDMRLIF 540
QNHRASYKYK DFGQMGLRLE AEFEKDFKEV FAIQETNGNS 580 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR001487; Bromodomain.
 IPR000770; SAND_dom.
 IPR010919; SAND_dom-like.
 IPR004865; Sp100.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00439; Bromodomain
 PF00628; PHD
 PF01342; SAND
 PF03172; Sp100 
SMART
 SM00297; BROMO
 SM00249; PHD
 SM00258; SAND 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51414; HSR
 PS50864; SAND
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 
PRINTS