CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007910
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic check point protein BFA1 
Protein Synonyms/Alias
 Cell cycle arrest protein BFA1 
Gene Name
 BFA1 
Gene Synonyms/Alias
 YJR053W; J1667 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
102FQEFQNKKDDFDDAIubiquitination[1]
328VQLNGPAKIKTIKQQacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 
Functional Description
 Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex. 
Sequence Annotation
 MOD_RES 317 317 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 574 AA 
Protein Sequence
MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP PPSSVGTSDT 60
GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN KKDDFDDAIK TNFHLRNGCR 120
TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS MMELKPKRKL SNSVTSRNLR SGNSVRFKKS 180
MPNLALVNPA IREEEEDEER EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE 240
GEAIDETISS NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT 300
QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN PKTMKWEGNE 360
NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA RATSRNQKVI GNMILDEQNL 420
RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA 480
AQARLRKYHS MRTLNGTTET PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII 540
SVDEETIMDE STVNSKRKSY MYEIRNMVIN STKD 574 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005096; F:GTPase activator activity; TAS:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031578; P:mitotic spindle orientation checkpoint; IMP:SGD. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS