CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020304
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Junctional adhesion molecule C 
Protein Synonyms/Alias
 JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3 
Gene Name
 JAM3 
Gene Synonyms/Alias
 UNQ859/PRO1868 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
97GRAEILGKTSLKIWNubiquitination[1]
148TPVCRVPKAVPVGKMubiquitination[1]
283KQDGESYKNPGKPDGubiquitination[2, 3, 4, 5]
305EEGDFRHKSSFVI**ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. 
Sequence Annotation
 DOMAIN 35 127 Ig-like V-type.
 DOMAIN 139 236 Ig-like C2-type.
 CARBOHYD 104 104 N-linked (GlcNAc...) (Potential).
 CARBOHYD 192 192 N-linked (GlcNAc...).
 CARBOHYD 198 198 N-linked (GlcNAc...); atypical.
 DISULFID 53 115 Potential.
 DISULFID 160 219 Potential.  
Keyword
 Alternative splicing; Angiogenesis; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 310 AA 
Protein Sequence
MALRRPPRLR LCARLPDFFL LLLFRGCLIG AVNLKSSNRT PVVQEFESVE LSCIITDSQT 60
SDPRIEWKKI QDEQTTYVFF DNKIQGDLAG RAEILGKTSL KIWNVTRRDS ALYRCEVVAR 120
NDRKEIDEIV IELTVQVKPV TPVCRVPKAV PVGKMATLHC QESEGHPRPH YSWYRNDVPL 180
PTDSRANPRF RNSSFHLNSE TGTLVFTAVH KDDSGQYYCI ASNDAGSARC EEQEMEVYDL 240
NIGGIIGGVL VVLAVLALIT LGICCAYRRG YFINNKQDGE SYKNPGKPDG VNYIRTDEEG 300
DFRHKSSFVI 310 
Gene Ontology
 GO:0044291; C:cell-cell contact zone; IDA:UniProtKB.
 GO:0030057; C:desmosome; IDA:UniProtKB.
 GO:0005615; C:extracellular space; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0033010; C:paranodal junction; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0043220; C:Schmidt-Lanterman incisure; IEA:Compara.
 GO:0005923; C:tight junction; IEA:Compara.
 GO:0002250; P:adaptive immune response; IEA:Compara.
 GO:0001525; P:angiogenesis; IDA:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007160; P:cell-matrix adhesion; IEA:Compara.
 GO:0030010; P:establishment of cell polarity; IEA:Compara.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0002523; P:leukocyte migration involved in inflammatory response; IEA:Compara.
 GO:0042552; P:myelination; IEA:Compara.
 GO:0002318; P:myeloid progenitor cell differentiation; IEA:Compara.
 GO:0001780; P:neutrophil homeostasis; IEA:Compara.
 GO:0090138; P:regulation of actin cytoskeleton organization by cell-cell adhesion; IEA:Compara.
 GO:0090022; P:regulation of neutrophil chemotaxis; IDA:UniProtKB.
 GO:0007286; P:spermatid development; IEA:Compara. 
Interpro
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR003598; Ig_sub2.
 IPR013106; Ig_V-set. 
Pfam
 PF07686; V-set 
SMART
 SM00409; IG
 SM00408; IGc2 
PROSITE
 PS50835; IG_LIKE 
PRINTS