CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019135
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leucine-rich repeat-containing protein 58 
Protein Synonyms/Alias
  
Gene Name
 LRRC58 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99GLRTLLAKNNRLGGPubiquitination[1, 2]
111GGPSALPKGLAQSPLubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
200YLVLCDNKIQSIPPQubiquitination[5]
300ASNCPNPKCGGVYFDubiquitination[2, 5]
314DCCVRQIKFVDFCGKubiquitination[2]
321KFVDFCGKYRLPLMHubiquitination[2, 5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 REPEAT 45 66 LRR 1.
 REPEAT 69 91 LRR 2.
 REPEAT 92 113 LRR 3.
 REPEAT 121 143 LRR 4.
 REPEAT 144 166 LRR 5.
 REPEAT 167 189 LRR 6.
 REPEAT 190 211 LRR 7.
 REPEAT 213 234 LRR 8.
 REPEAT 236 256 LRR 9.
 MOD_RES 24 24 Phosphoserine.  
Keyword
 Complete proteome; Leucine-rich repeat; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 371 AA 
Protein Sequence
MEEAGAAVVT AGEAELNWSR LSVSTETLES ELEARGEERR GAREALLRLL LPHNRLVSLP 60
RALGSGFPHL QLLDVSGNAL TALGPELLAL RGLRTLLAKN NRLGGPSALP KGLAQSPLCR 120
SLQVLNLSGN CFQEVPASLL ELRALQTLSL GGNQLQSIPA EIENLQSLEC LYLGGNFIKE 180
IPPELGNLPS LNYLVLCDNK IQSIPPQLSQ LHSLRSLSLH NNLLTYLPRE ILNLIHLEEL 240
SLRGNPLVVR FVRDLTYDPP TLLELAARTI KIRNISYTPY DLPGNLLRYL GSASNCPNPK 300
CGGVYFDCCV RQIKFVDFCG KYRLPLMHYL CSPECSSPCS SASHSSTSQS ESDSEDEASV 360
AARRMQKVLL G 371 
Gene Ontology
  
Interpro
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR003591; Leu-rich_rpt_typical-subtyp. 
Pfam
 PF12799; LRR_4 
SMART
 SM00369; LRR_TYP 
PROSITE
 PS51450; LRR 
PRINTS