UniProt Accession

 FABP5_HUMAN; Q01469; B2R4K0 

Genbank Protein ID

 M94856; BT007449; AK311856; CH471068; BC019385; BC070303 

Genbank Nucleotide ID

 AAA58467.1; AAP36117.1; BAG34797.1; EAW87088.1; AAH19385.1; AAH70303.1 

Protein Name

 Fatty acid-binding protein, epidermal 

Protein Synonyms/Alias

 Epidermal-type fatty acid-binding protein; E-FABP; Fatty acid-binding protein 5; Psoriasis-associated fatty acid-binding protein homolog; PA-FABP 

Gene Name

 FABP5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	RWRLVDSKGFDEYMK	acetylation	[1, 2, 3, 4]
17	RWRLVDSKGFDEYMK	ubiquitination	[4, 5, 6, 7]
24	KGFDEYMKELGVGIA	ubiquitination	[4, 5, 6, 7, 8, 9, 10]
34	GVGIALRKMGAMAKP	ubiquitination	[4, 6, 7, 9]
61	IKTESTLKTTQFSCT	ubiquitination	[5, 7]
72	FSCTLGEKFEETTAD	ubiquitination	[7]
82	ETTADGRKTQTVCNF	ubiquitination	[5, 7]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. 

Sequence Annotation

 REGION 129 131 Fatty acid binding (By similarity).
 BINDING 109 109 Fatty acid (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 17 17 N6-acetyllysine.
 MOD_RES 131 131 Phosphotyrosine.
 DISULFID 120 127  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Lipid-binding; Phosphoprotein; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 135 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; TAS:UniProtKB.
 GO:0005504; F:fatty acid binding; TAS:ProtInc.
 GO:0005215; F:transporter activity; IEA:InterPro.
 GO:0008544; P:epidermis development; TAS:ProtInc.
 GO:0006006; P:glucose metabolic process; IEA:Compara.
 GO:0015758; P:glucose transport; IEA:Compara.
 GO:0006629; P:lipid metabolic process; TAS:ProtInc.
 GO:0006656; P:phosphatidylcholine biosynthetic process; IEA:Compara. 

Interpro

 IPR012674; Calycin.
 IPR011038; Calycin-like.
 IPR000463; Fatty_acid-bd.
 IPR000566; Lipocln_cytosolic_FA-bd_dom. 

Pfam

 PF00061; Lipocalin 

SMART

  

PROSITE

 PS00214; FABP 

PRINTS

 PR00178; FATTYACIDBP.