CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009232
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin, cytoplasmic 1 
Protein Synonyms/Alias
 Beta-actin; Actin, cytoplasmic 1, N-terminally processed 
Gene Name
 Actb 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
50VMVGMGQKDSYVGDEacetylation[1]
50VMVGMGQKDSYVGDEubiquitination[2]
61VGDEAQSKRGILTLKacetylation[1]
61VGDEAQSKRGILTLKubiquitination[2]
68KRGILTLKYPIEHGIacetylation[1]
113TEAPLNPKANREKMTacetylation[1]
113TEAPLNPKANREKMTubiquitination[2]
291KCDVDIRKDLYANTVubiquitination[2]
315GIADRMQKEITALAPacetylation[1]
315GIADRMQKEITALAPubiquitination[2]
326ALAPSTMKIKIIAPPacetylation[1]
326ALAPSTMKIKIIAPPubiquitination[2]
328APSTMKIKIIAPPERubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylaspartate; in Actin, cytoplasmic
 MOD_RES 44 44 Methionine sulfoxide (By similarity).
 MOD_RES 73 73 Tele-methylhistidine (By similarity).
 MOD_RES 218 218 Nitrated tyrosine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methylation; Nitration; Nucleotide-binding; Oxidation; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 375 AA 
Protein Sequence
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS 60
KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT 120
QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL 180
AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY 240
ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS 300
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ 360
EYDESGPSIV HRKCF 375 
Gene Ontology
 GO:0030424; C:axon; IDA:RGD.
 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0070688; C:MLL5-L complex; IEA:Compara.
 GO:0035267; C:NuA4 histone acetyltransferase complex; IEA:Compara.
 GO:0014069; C:postsynaptic density; IDA:RGD.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0030529; C:ribonucleoprotein complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0007409; P:axonogenesis; IDA:RGD. 
Interpro
 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR004001; Actin_CS. 
Pfam
 PF00022; Actin 
SMART
 SM00268; ACTIN 
PROSITE
 PS00406; ACTINS_1
 PS00432; ACTINS_2
 PS01132; ACTINS_ACT_LIKE 
PRINTS
 PR00190; ACTIN.