UniProt Accession

 GPA2_YEAST; P10823; D3DLR9 

Genbank Protein ID

 J03609; U18778; BK006939 

Genbank Nucleotide ID

 AAA34651.1; AAB64553.1; DAA07673.1 

Protein Name

 Guanine nucleotide-binding protein alpha-2 subunit 

Protein Synonyms/Alias

 GP2-alpha 

Gene Name

 GPA2 

Gene Synonyms/Alias

 SSP101; YER020W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
52	NTANQQEKQQQRQQQ	ubiquitination	[1]
122	ASSGSNDKELKVLLL	ubiquitination	[1]
394	TGGSDINKAAKYILW	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Alpha subunit of the heterotrimeric guanine nucleotide- binding protein (G protein) involved in glucose-induced cAMP signaling. Binds to its cognate transmembrane receptor GPR1, which senses extracellular carbon sources, and activates cAMP-PKA signaling and governs diploid pseudohyphal differentiation and haploid invasive growth. The G protein beta-mimic proteins GPB1 and GPB2 inhibit GPA2-GPR1 coupling, probably to reduce signaling in the absence of glucose. 

Sequence Annotation

 NP_BIND 130 137 GTP (By similarity).
 NP_BIND 270 276 GTP (By similarity).
 NP_BIND 296 300 GTP (By similarity).
 NP_BIND 365 368 GTP (By similarity).
 METAL 137 137 Magnesium (By similarity).
 METAL 276 276 Magnesium (By similarity).
 BINDING 420 420 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine.
 LIPID 4 4 S-palmitoyl cysteine.  

Keyword

 Cell membrane; Complete proteome; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Palmitate; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 449 AA 

Protein Sequence

Gene Ontology

 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0007189; P:adenylate cyclase-activating G-protein coupled receptor signaling pathway; IMP:SGD.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0010255; P:glucose mediated signaling pathway; IMP:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD.
 GO:0008361; P:regulation of cell size; IMP:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD. 

Interpro

 IPR002975; Fungi_Gprotein_alpha.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR01241; GPROTEINAFNG.