CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000539
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic 
Protein Synonyms/Alias
 GPD-C; GPDH-C 
Gene Name
 Gpd1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
49EEDIGGRKLTEIINTubiquitination[1]
62NTQHENVKYLPGHKLacetylation[2]
106GKICDQLKGHLKANTacetylation[2]
120TIGISLIKGIDEGPNacetylation[2]
282EAFARTGKSIEQLEKacetylation[2]
289KSIEQLEKEMLNGQKacetylation[2]
296KEMLNGQKLQGPQTAacetylation[2]
313LHSILQHKGLVDKFPacetylation[2]
318QHKGLVDKFPLFTAVacetylation[2]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
  
Sequence Annotation
 NP_BIND 10 15 NAD (By similarity).
 REGION 269 270 Substrate binding (By similarity).
 ACT_SITE 204 204 Proton acceptor (Potential).
 BINDING 41 41 NAD (By similarity).
 BINDING 97 97 NAD (By similarity).
 BINDING 120 120 Substrate (By similarity).
 BINDING 153 153 NAD; via amide nitrogen (By similarity).
 BINDING 269 269 NAD (By similarity).
 BINDING 296 296 NAD; via amide nitrogen (By similarity).
 BINDING 298 298 NAD (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 349 AA 
Protein Sequence
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 60
VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK 120
GIDEGPNGLK LISEVIGESL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKEL 180
MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 240
LFCSGSVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 300
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM 349 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IDA:RGD.
 GO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0005975; P:carbohydrate metabolic process; IDA:RGD.
 GO:0071320; P:cellular response to cAMP; IEA:Compara.
 GO:0071356; P:cellular response to tumor necrosis factor; IEA:Compara.
 GO:0006094; P:gluconeogenesis; IEA:Compara.
 GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
 GO:0006072; P:glycerol-3-phosphate metabolic process; IDA:RGD.
 GO:0046486; P:glycerolipid metabolic process; IDA:RGD.
 GO:0006116; P:NADH oxidation; IDA:RGD. 
Interpro
 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR006168; G3P_DH_NAD-dep.
 IPR006109; G3P_DH_NAD-dep_C.
 IPR017751; G3P_DH_NAD-dep_euk.
 IPR011128; G3P_DH_NAD-dep_N.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF07479; NAD_Gly3P_dh_C
 PF01210; NAD_Gly3P_dh_N 
SMART
  
PROSITE
 PS00957; NAD_G3PDH 
PRINTS
 PR00077; GPDHDRGNASE.