UniProt Accession

 NB5R3_MOUSE; Q9DCN2 

Genbank Protein ID

 AF332059; AF332060; AK002640; BC004760; BC032013; BC043074 

Genbank Nucleotide ID

 AAK56088.1; AAK56089.1; BAB22252.1; AAH04760.1; AAH32013.1; AAH43074.1 

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

 B5R; Cytochrome b5 reductase; Diaphorase-1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form 

Gene Name

 Cyb5r3 

Gene Synonyms/Alias

 Dia1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
42	TLENPDIKYPLRLID	acetylation	[1, 2]
42	TLENPDIKYPLRLID	ubiquitination	[3]
50	YPLRLIDKEVISPDT	acetylation	[2]
50	YPLRLIDKEVISPDT	ubiquitination	[3]
115	LVVKVYFKDTHPKFP	acetylation	[2]
115	LVVKVYFKDTHPKFP	ubiquitination	[3]
120	YFKDTHPKFPAGGKM	acetylation	[1, 2]
120	YFKDTHPKFPAGGKM	ubiquitination	[3]
126	PKFPAGGKMSQYLEN	ubiquitination	[3]
135	SQYLENMKIGDTIEF	ubiquitination	[3]
154	GLLVYQGKGKFAIRA	ubiquitination	[3]
173	NPVVRTVKSVGMIAG	ubiquitination	[3]
196	QVIRAVLKDPNDHTV	acetylation	[2]
234	NEHSARFKLWYTVDK	ubiquitination	[3]
241	KLWYTVDKAPDAWDY	ubiquitination	[3]
295	LERVGHPKERCFTF*	acetylation	[4]

Reference

 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). 

Sequence Annotation

 DOMAIN 40 152 FAD-binding FR-type.
 NP_BIND 132 147 FAD (By similarity).
 NP_BIND 171 206 FAD (By similarity).
 MOD_RES 42 42 N6-acetyllysine.
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 120 120 N6-acetyllysine.
 MOD_RES 130 130 Phosphotyrosine.
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 Acetylation; Alternative promoter usage; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0016208; F:AMP binding; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:EC.
 GO:0071949; F:FAD binding; IEA:Compara.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.