CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006956
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADH-cytochrome b5 reductase 1 
Protein Synonyms/Alias
 Microsomal cytochrome b reductase; P35 
Gene Name
 CBR1 
Gene Synonyms/Alias
 CBR; CBR5; YIL043C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
86IKANINGKDITRSYTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes. Plays a role in bud morphology. 
Sequence Annotation
 DOMAIN 38 142 FAD-binding FR-type.
 NP_BIND 122 137 FAD (By similarity).
 NP_BIND 148 180 FAD (By similarity).  
Keyword
 Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 284 AA 
Protein Sequence
MAIDAQKLVV VIVIVVVPLL FKFIIGPKTK PVLDPKRNDF QSFPLVEKTI LTHNTSMYKF 60
GLPHADDVLG LPIGQHIVIK ANINGKDITR SYTPTSLDGD TKGNFELLVK SYPTGNVSKM 120
IGELKIGDSI QIKGPRGNYH YERNCRSHLG MIAGGTGIAP MYQIMKAIAM DPHDTTKVSL 180
VFGNVHEEDI LLKKELEALV AMKPSQFKIV YYLDSPDRED WTGGVGYITK DVIKEHLPAA 240
TMDNVQILIC GPPAMVASVR RSTVDLGFRR SKPLSKMEDQ VFVF 284 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IDA:SGD. 
Interpro
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.