CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030032
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin light chain kinase, smooth muscle 
Protein Synonyms/Alias
 Myosin, light polypeptide kinase 
Gene Name
 Mylk 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1633NKTGTRIKLIDFGLAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1950 AA 
Protein Sequence
MSGQLPGKTM GDVKLFASSH MSKTSHSVDP SKVSSMPLTE APAFILPPRN LCVKEGATAK 60
FEGRVRGYPE PQVTWHRKGQ AITNGGRFLL DCGVRGTFSL VIHTVREEDK GKYTCEASNG 120
SGARQVTVEL TVEGNSMKKR DQPVLSKASG FPGETRPSIW GECPPKFATK LGRAVVKEGQ 180
MGRFSCKITG RPPPQVTWLK GNVPLQPSAR VSMSEKNGMQ ILEIRGVTRD DLGVYTCMVV 240
NGSGKASMSA ELSIPGLDNA ARLAVRGTKA PSPDIRKEVT NGVSKDPETV AESKNCPSPQ 300
RSGSSARATN SHLKSPQEPK PKLCEDAPRK VPQSSILQKS TSTITLQALK VQPEARVPAI 360
GSFSPGEDRK SLAAPQQATL PTRQSSLGGS VGNKFVTGNI PRESQRESTF PRFESQPQSQ 420
EVTEGQTVKF ICEVSGIPKP DVGWFLEGIP VRRREGITEV YEDGVSHHLC LLRARTRDSG 480
RYSCTASNSL GQVSCSWSLL VDRPNLAQTA PSFSSVLKDS VVIEGQDFVL RCSVQGTPAP 540
RVTWLLNGQP IQFAHSICEA GVAELHIQDA LPEDRGTYTC LAENAMGQVS CSATVTVQEK 600
KGEGEREHRL SPARSKPIAP IFLQGLSDLK VMDGSQVTMT VQVSGNPPPE VIWLHDGNEI 660
QESEDFHFEQ KGGWHSLCIQ EVFPEDTGTY TCEAWNSAGE VRTRAVLTVQ EPHDGTQPWF 720
ISKPRSVTAT LGQSVLISCA IAGDPFPTVH WLRDGRALSK DSGHFELLQN EDVFTLVLKN 780
VQPWHAGQYE ILLKNRVGEC SCQVSLMLHN SPSRAPPRGR EPASCEGLCG GGGVGAHGDG 840
DRHGTLRPCW PARGQGWPEE EDGEDVRGLL KRRVETRLHT EEAIRQQEVG QLDFRDLLGK 900
KVSTKTVSED DLKDIPAEQM DFRANLQRQV KPKTISEEER KVHSPQQVDF RSVLAKKGTP 960
KTPVPEKAPP KAATPDFRSV LGGKKKSPSE NGGNSAEVLN VKAGESPTPA GDAQAIGALK 1020
PVGNAKPAET PKPIGNAKPT ETLKPVGNTK PAETLKPIAN AQPSGSLKPV TNAQPAEPQK 1080
PVGNAKSAET SKPAGKEEVK EVKNDVNCKK GQVGATGNEK RPESQGSAPV FKEKLQDVHV 1140
AEGEKLLLQC QVISDPPATV TWSLNGKTLK TTKFIVLAQE GSRFSVSIEK ALPEDRGLYK 1200
CVAKNSAGQA ECSCQVTVDD AQTSENTKAP EMKSRRPKSS LPPVLGTESD ATVKKKPAPK 1260
TPTKAAMPPQ IIQFPEDQKV RAGEPVELFG KVAGTQPITC KWMKFRKQIQ ESEHIKVENG 1320
ESGSKLTILA ARQEHCGCYT LVVENKLGSR QAQVNLTVVD KPDPPAGTPC ASDIRSSSLT 1380
LSWYGSSYDG GSAVQSYNVE IWDTEDKVWK ELATCRSTSF NVQDLLPDRE YKFRVRAVNV 1440
YGTSEPSQES ELTAVGEKPE EPKDEVEVSD DDEKEPEVDY RTVTVNTEQK VSDVYDIEER 1500
LGSGKFGQVF RLVEKKTGKI WAGKFFKAYS AKEKDNIRQE ISIMNCLHHP KLVQCVDAFE 1560
EKANIVMVLE IVSGGELFER IIDEDFELTE RECIKYMRQI SEGVEYIHKQ GIVHLDLKPE 1620
NIMCVNKTGT RIKLIDFGLA RRLENAGSLK VLFGTPEFVA PEVINYEPIG YATDMWSIGV 1680
ICYILVSGLS PFMGDNDNET LANVTSATWD FDDEAFDEIS DDAKDFISNL LKKDMKNRLD 1740
CTQCLQHPWL MKDTKNMEAK KLSKDRMKKY MARRKWQKTG NAVRAIGRLS SMAMISGLSG 1800
RKSSTGSPTS PINAEKLESE DDVSQAFLEA VAEEKPHVKP YFSKTIRDLE VVEGSAARFD 1860
CKIEGYPDPE VVWFKDDQSI RESRHFQIDY DEDGNCSLII SDVCGDDDAK YTCKAVNSLG 1920
EATCTAELIV ETMEEGEGEE GGEEEEEEEE 1950 
Gene Ontology
 GO:0032154; C:cleavage furrow; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0001725; C:stress fiber; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004687; F:myosin light chain kinase activity; IEA:Compara.
 GO:0060414; P:aorta smooth muscle tissue morphogenesis; IEA:Compara.
 GO:0032060; P:bleb assembly; IEA:Compara.
 GO:0071476; P:cellular hypotonic response; IEA:Compara.
 GO:0051928; P:positive regulation of calcium ion transport; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0090303; P:positive regulation of wound healing; IEA:Compara. 
Interpro
 IPR003961; Fibronectin_type3.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR013098; Ig_I-set.
 IPR003599; Ig_sub.
 IPR003598; Ig_sub2.
 IPR011009; Kinase-like_dom.
 IPR020675; Myosin_light_ch_kinase-rel.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00041; fn3
 PF07679; I-set
 PF00069; Pkinase 
SMART
 SM00060; FN3
 SM00409; IG
 SM00408; IGc2
 SM00220; S_TKc 
PROSITE
 PS50853; FN3
 PS50835; IG_LIKE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS