UniProt Accession

 IDH3A_HUMAN; P50213; D3DW83; Q9H3X0 

Genbank Protein ID

 U07681; AL442090; CH471136; CH471136; BC021967 

Genbank Nucleotide ID

 AAA85639.1; CAC09449.1; EAW99181.1; EAW99182.1; AAH21967.1 

Protein Name

 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial 

Protein Synonyms/Alias

 Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha 

Gene Name

 IDH3A 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
58	MKIFDAAKAPIQWEE	ubiquitination	[1, 2, 3, 4, 5]
77	AIQGPGGKWMIPSEA	ubiquitination	[1, 2, 4, 5]
100	MGLKGPLKTPIAAGH	ubiquitination	[1, 3, 5]
116	SMNLLLRKTFDLYAN	ubiquitination	[3]
177	LITEGASKRIAEFAF	ubiquitination	[3]
200	SNVTAVHKANIMRMS	ubiquitination	[3]
214	SDGLFLQKCREVAES	ubiquitination	[3]
223	REVAESCKDIKFNEM	ubiquitination	[3]
336	AACFATIKDGKSLTK	ubiquitination	[3]
343	KDGKSLTKDLGGNAK	acetylation	[6]
343	KDGKSLTKDLGGNAK	ubiquitination	[3]
350	KDLGGNAKCSDFTEE	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

  

Sequence Annotation

 METAL 233 233 Magnesium or manganese (By similarity).
 METAL 257 257 Magnesium or manganese (By similarity).
 METAL 261 261 Magnesium or manganese (By similarity).
 BINDING 115 115 Substrate (By similarity).
 BINDING 125 125 Substrate (By similarity).
 BINDING 146 146 Substrate (By similarity).
 BINDING 233 233 Substrate (By similarity).
 MOD_RES 343 343 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 366 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0005975; P:carbohydrate metabolic process; NAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR004434; Isocitrate_DH_NAD.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS