CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003227
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal small subunit pseudouridine synthase A 
Protein Synonyms/Alias
 16S pseudouridylate 516 synthase; 16S rRNA pseudouridine(516) synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A 
Gene Name
 rsuA 
Gene Synonyms/Alias
 yejD; b2183; JW2171 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
5***MRLDKFIAQQLGacetylation[1]
40IVRNAAFKLLPEHDVacetylation[1]
130SPRHHCEKTYLVTLEacetylation[1]
150DTAEQFAKGVQLHNEacetylation[1]
158GVQLHNEKDLTKPAVacetylation[1]
162HNEKDLTKPAVLEVIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA. 
Sequence Annotation
 DOMAIN 1 68 S4 RNA-binding.
 ACT_SITE 102 102 Nucleophile (Probable).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Reference proteome; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 231 AA 
Protein Sequence
MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD GNPLAQQHGP 60
RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR LDIDTTGLVL MTDDGQWSHR 120
ITSPRHHCEK TYLVTLESPV ADDTAEQFAK GVQLHNEKDL TKPAVLEVIT PTQVRLTISE 180
GRYHQVKRMF AAVGNHVVEL HRERIGGITL DADLAPGEYR PLTEEEIASV V 231 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IDA:EcoCyc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IMP:EcoCyc. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
 IPR018496; PsdUridine_synth_RsuA/RluB_CS.
 IPR006145; PsdUridine_synth_RsuA/RluD.
 IPR002942; S4_RNA-bd. 
Pfam
 PF00849; PseudoU_synth_2
 PF01479; S4 
SMART
 SM00363; S4 
PROSITE
 PS01149; PSI_RSU
 PS50889; S4 
PRINTS