CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018891
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Interferon regulatory factor 7 
Protein Synonyms/Alias
 IRF-7 
Gene Name
 IRF7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92TAERAGWKTNFRCALacetylation[1]
120GDPADPHKVYALSREubiquitination[2]
341PAELPDQKQLRYTEEubiquitination[3]
446SAGRPKEKSLVLVKLsumoylation[4]
452EKSLVLVKLEPWLCRsumoylation[4]
Reference
 [1] Acetylation of interferon regulatory factor-7 by p300/CREB-binding protein (CBP)-associated factor (PCAF) impairs its DNA binding.
 Caillaud A, Prakash A, Smith E, Masumi A, Hovanessian AG, Levy DE, Marié I.
 J Biol Chem. 2002 Dec 20;277(51):49417-21. [PMID: 12374802]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7.
 Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 3rd, Ozato K, Zhu F.
 J Immunol. 2011 Nov 1;187(9):4754-63. [PMID: 21940674] 
Functional Description
 Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88- independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. 
Sequence Annotation
 DNA_BIND 11 126 IRF tryptophan pentad repeat.
 MOD_RES 92 92 N6-acetyllysine; by KAT2A and KAT2B.
 MOD_RES 477 477 Phosphoserine; by TBK1 and IKKE.
 MOD_RES 479 479 Phosphoserine; by TBK1 and IKKE.
 CROSSLNK 444 444 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 446 446 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Antiviral defense; Complete proteome; Cytoplasm; DNA-binding; Host-virus interaction; Immunity; Innate immunity; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MALAPERAAP RVLFGEWLLG EISSGCYEGL QWLDEARTCF RVPWKHFARK DLSEADARIF 60
KAWAVARGRW PPSSRGGGPP PEAETAERAG WKTNFRCALR STRRFVMLRD NSGDPADPHK 120
VYALSRELCW REGPGTDQTE AEAPAAVPPP QGGPPGPFLA HTHAGLQAPG PLPAPAGDKG 180
DLLLQAVQQS CLADHLLTAS WGADPVPTKA PGEGQEGLPL TGACAGGPGL PAGELYGWAV 240
ETTPSPGPQP AALTTGEAAA PESPHQAEPY LSPSPSACTA VQEPSPGALD VTIMYKGRTV 300
LQKVVGHPSC TFLYGPPDPA VRATDPQQVA FPSPAELPDQ KQLRYTEELL RHVAPGLHLE 360
LRGPQLWARR MGKCKVYWEV GGPPGSASPS TPACLLPRNC DTPIFDFRVF FQELVEFRAR 420
QRRGSPRYTI YLGFGQDLSA GRPKEKSLVL VKLEPWLCRV HLEGTQREGV SSLDSSSLSL 480
CLSSANSLYD DIECFLMELE QPA 503 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0010008; C:endosome membrane; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000982; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:UniProtKB.
 GO:0019043; P:establishment of viral latency; TAS:UniProtKB.
 GO:0016064; P:immunoglobulin mediated immune response; IEA:Compara.
 GO:0032607; P:interferon-alpha production; ISS:UniProtKB.
 GO:0032608; P:interferon-beta production; ISS:UniProtKB.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0039530; P:MDA-5 signaling pathway; TAS:UniProtKB.
 GO:2000110; P:negative regulation of macrophage apoptotic process; TAS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0032727; P:positive regulation of interferon-alpha production; TAS:UniProtKB.
 GO:0032728; P:positive regulation of interferon-beta production; TAS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0060340; P:positive regulation of type I interferon-mediated signaling pathway; IEA:Compara.
 GO:0002819; P:regulation of adaptive immune response; IDA:UniProtKB.
 GO:0045655; P:regulation of monocyte differentiation; TAS:UniProtKB.
 GO:0034124; P:regulation of MyD88-dependent toll-like receptor signaling pathway; ISS:UniProtKB.
 GO:0034127; P:regulation of MyD88-independent toll-like receptor signaling pathway; ISS:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; TAS:UniProtKB.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0045351; P:type I interferon biosynthetic process; IEA:Compara.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 
Interpro
 IPR019817; Interferon_reg_fac_CS.
 IPR001346; Interferon_reg_fact_DNA-bd_dom.
 IPR019471; Interferon_reg_factor-3.
 IPR017855; SMAD_dom-like.
 IPR008984; SMAD_FHA_domain.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00605; IRF
 PF10401; IRF-3 
SMART
 SM00348; IRF 
PROSITE
 PS00601; IRF_1
 PS51507; IRF_2 
PRINTS
 PR00267; INTFRNREGFCT.