CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006499
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Insulin receptor substrate 1 
Protein Synonyms/Alias
 IRS-1 
Gene Name
 Irs1 
Gene Synonyms/Alias
 Irs-1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
315PASMVGGKPGSFRVRacetylation[1]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). 
Sequence Annotation
 DOMAIN 12 115 PH.
 DOMAIN 155 259 IRS-type PTB.
 REGION 3 133 Mediates interaction with PHIP (By
 REGION 891 893 GRB2-binding (By similarity).
 MOTIF 460 463 YXXM motif 1.
 MOTIF 546 549 YXXM motif 2.
 MOTIF 608 611 YXXM motif 3.
 MOTIF 628 631 YXXM motif 4.
 MOTIF 658 661 YXXM motif 5.
 MOTIF 727 730 YXXM motif 6.
 MOTIF 935 938 YXXM motif 7.
 MOTIF 983 986 YXXM motif 8.
 MOTIF 1006 1009 YXXM motif 9.
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 99 99 Phosphoserine; by CK2 (By similarity).
 MOD_RES 265 265 Phosphoserine; by RPS6KB1 (By
 MOD_RES 302 302 Phosphoserine; by RPS6KB1 (By
 MOD_RES 307 307 Phosphoserine; by IKKB and MAPK8 (By
 MOD_RES 324 324 Phosphoserine.
 MOD_RES 340 340 Phosphoserine.
 MOD_RES 343 343 Phosphoserine (By similarity).
 MOD_RES 460 460 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 608 608 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 628 628 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 632 632 Phosphoserine; by RPS6KB1 and ROCK2
 MOD_RES 658 658 Phosphotyrosine (By similarity).
 MOD_RES 789 789 Phosphoserine; by AMPK and SIK2.
 MOD_RES 891 891 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 935 935 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 983 983 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 1097 1097 Phosphoserine; by RPS6KB1 and PKC/PRKCQ
 MOD_RES 1139 1139 Phosphoserine.
 MOD_RES 1173 1173 Phosphotyrosine; by INSR (By similarity).
 MOD_RES 1214 1214 Phosphoserine.
 MOD_RES 1220 1220 Phosphotyrosine; by INSR (By similarity).  
Keyword
 3D-structure; Complete proteome; Phosphoprotein; Reference proteome; Repeat; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1233 AA 
Protein Sequence
MASPPDTDGF SDVRKVGYLR KPKSMHKRFF VLRAASEAGG PARLEYYENE KKWRHKSSAP 60
KRSIPLESCF NINKRADSKN KHLVALYTRD EHFAIAADSE AEQDSWYQAL LQLHNRAKAH 120
HDGAGGGCGG SCSGSSGVGE AGEDLSYDTG PGPAFKEVWQ VILKPKGLGQ TKNLIGIYRL 180
CLTSKTISFV KLNSEAAAVV LQLMNIRRCG HSENFFFIEV GRSAVTGPGE FWMQVDDSVV 240
AQNMHETILE AMRAMSDEFR PRSKSQSSSS CSNPISVPLR RHHLNNPPPS QVGLTRRSRT 300
ESITATSPAS MVGGKPGSFR VRASSDGEGT MSRPASVDGS PVSPSTNRTH AHRHRGSSRL 360
HPPLNHSRSI PMPSSRCSPS ATSPVSLSSS STSGHGSTSD CLFPRRSSAS VSGSPSDGGF 420
ISSDEYGSSP CDFRSSFRSV TPDSLGHTPP ARGEEELSNY ICMGGKGAST LAAPNGHYIL 480
SRGGNGHRYI PGANLGTSPA LPGDEAAGAA DLDNRFRKRT HSAGTSPTIS HQKTPSQSSV 540
ASIEEYTEMM PAAYPPGGGS GGRLPGYRHS AFVPTHSYPE EGLEMHHLER RGGHHRPDTS 600
NLHTDDGYMP MSPGVAPVPS NRKGNGDYMP MSPKSVSAPQ QIINPIRRHP QRVDPNGYMM 660
MSPSGSCSPD IGGGSSSSSS ISAAPSGSSY GKPWTNGVGG HHTHALPHAK PPVESGGGKL 720
LPCTGDYMNM SPVGDSNTSS PSECYYGPED PQHKPVLSYY SLPRSFKHTQ RPGEPEEGAR 780
HQHLRLSSSS GRLRYTATAE DSSSSTSSDS LGGGYCGARP ESSLTHPHHH VLQPHLPRKV 840
DTAAQTNSRL ARPTRLSLGD PKASTLPRVR EQQQQQQSSL HPPEPKSPGE YVNIEFGSGQ 900
PGYLAGPATS RSSPSVRCPP QLHPAPREET GSEEYMNMDL GPGRRATWQE SGGVELGRIG 960
PAPPGSATVC RPTRSVPNSR GDYMTMQIGC PRQSYVDTSP VAPVSYADMR TGIAAEKASL 1020
PRPTGAAPPP SSTASSSASV TPQGATAEQA THSSLLGGPQ GPGGMSAFTR VNLSPNHNQS 1080
AKVIRADTQG CRRRHSSETF SAPTRAGNTV PFGAGAAVGG SGGGGGGGSE DVKRHSSASF 1140
ENVWLRPGDL GGVSKESAPV CGAAGGLEKS LNYIDLDLAK EHSQDCPSQQ QSLPPPPPHQ 1200
PLGSNEGNSP RRSSEDLSNY ASISFQKQPE DRQ 1233 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0005158; F:insulin receptor binding; ISS:UniProtKB.
 GO:0005159; F:insulin-like growth factor receptor binding; ISS:UniProtKB.
 GO:0043548; F:phosphatidylinositol 3-kinase binding; ISS:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0019901; F:protein kinase binding; IDA:MGI.
 GO:0042169; F:SH2 domain binding; ISS:UniProtKB.
 GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; ISS:UniProtKB.
 GO:0048009; P:insulin-like growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0016042; P:lipid catabolic process; IMP:MGI.
 GO:0030879; P:mammary gland development; IGI:MGI.
 GO:0030335; P:positive regulation of cell migration; IGI:MGI.
 GO:0002053; P:positive regulation of mesenchymal cell proliferation; IGI:MGI.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IMP:MGI.
 GO:0043491; P:protein kinase B signaling cascade; IGI:MGI.
 GO:0034504; P:protein localization to nucleus; IDA:MGI.
 GO:0010468; P:regulation of gene expression; IMP:MGI. 
Interpro
 IPR002404; Insln_rcpt_S1.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF02174; IRS
 PF00169; PH 
SMART
 SM00233; PH
 SM00310; PTBI 
PROSITE
 PS51064; IRS_PTB
 PS50003; PH_DOMAIN 
PRINTS
 PR00628; INSULINRSI.