CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011181
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable GTPase-activating protein GYL1 
Protein Synonyms/Alias
 GYP5-like protein 1 
Gene Name
 GYL1 
Gene Synonyms/Alias
 APP2; YMR192W; YM9646.04 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
498NSRDHLLKMGFDDMLsumoylation[1]
Reference
 [1] A proteomic strategy for gaining insights into protein sumoylation in yeast.
 Denison C, Rudner AD, Gerber SA, Bakalarski CE, Moazed D, Gygi SP.
 Mol Cell Proteomics. 2005 Mar;4(3):246-54. [PMID: 15542864] 
Functional Description
 Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis. 
Sequence Annotation
 DOMAIN 297 477 Rab-GAP TBC.
 MOD_RES 17 17 Phosphothreonine.
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 73 73 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 CROSSLNK 498 498 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Exocytosis; GTPase activation; Isopeptide bond; Phosphoprotein; Protein transport; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 720 AA 
Protein Sequence
MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK VEMATTNASS 60
NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP SDDLSQQLET EESKVEEALK 120
RITSPPLPPR ADCIEESASA LKSSLPPVLA GNKNDQAPLD RPQLPPRQVV NAETLHLKAP 180
HGNATPSKSP TSAVGNSSSS TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS 240
IKCDLDKNIL NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA 300
AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE YVKRISNVLK 360
AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM KVYGLRLLFL PSASEIDILC 420
YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS 480
IMRILATLLS NSRDHLLKMG FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD 540
SMMKVAITPK TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE 600
HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP NDLKKDLEET 660
MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY SNSKNNPLGA GWSGFKKVFK 720 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0005798; C:Golgi-associated vesicle; IDA:SGD.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IGI:SGD.
 GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0017157; P:regulation of exocytosis; IGI:SGD. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS