CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024112
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Large proline-rich protein BAG6 
Protein Synonyms/Alias
 BAG family molecular chaperone regulator 6; BCL2-associated athanogene 6; BAG-6; BAG6; HLA-B-associated transcript 3; Protein Scythe 
Gene Name
 Bag6 
Gene Synonyms/Alias
 Bat3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
40VGAQMNVKEFKEHIAubiquitination[1]
56SVSIPSEKQRLIYQGubiquitination[1]
71RVLQDDKKLQEYNVGubiquitination[1]
80QEYNVGGKVIHLVERubiquitination[1]
1055IQSQRKVKPQPPLSDubiquitination[1]
1074GMPAKRRKTMQGEGPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane (By similarity). Participates in endoplasmic reticulum stress- induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. 
Sequence Annotation
 DOMAIN 17 92 Ubiquitin-like.
 REPEAT 237 271 1.
 REPEAT 416 444 2.
 REPEAT 597 624 3.
 REPEAT 630 658 4.
 REGION 237 658 4 X 29 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 986 986 Phosphoserine.
 MOD_RES 995 995 Phosphoserine.
 MOD_RES 1103 1103 Phosphoserine (By similarity).
 MOD_RES 1139 1139 Phosphoserine (By similarity).  
Keyword
 Acetylation; Apoptosis; Chaperone; Chromatin regulator; Complete proteome; Cytoplasm; Differentiation; Immunity; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spermatogenesis; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1154 AA 
Protein Sequence
MEPSDSASTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 60
YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTQLPSGASS GTGSASATHG GAPLPGTRGP 120
GASVHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 180
SRMECRGGTQ AQASQPPPQT PQTVASETVA LNSQTSEPVE SEAPPREPME SEEMEERPPT 240
QTPELAPSGP APAGPAPAGP APAPETNAPN HPSPAEHVEV LQELQRLQRR LQPFLQRYCE 300
VLGAAATTDY NNNHEGREED QRLINLVGES LRLLGNTFVA LSDLRCNLAC APPRHLHVVR 360
PMSHYTTPMV LQQAAIPIQI NVGTTVTMTG NGARPPPAPG AEAATPGSAQ ATSLPPSSTT 420
VDSSTEGAPP PGPAPPPASS HPRVIRISHQ SVEPVVMMHM NIQDSGAQPG GVPSAPTGPL 480
GPPGHGQTLG QQVPGFPTAP TRVVIARPTP PQARPSHPGG PPVSGALQGA GLGTNTSLAQ 540
MVSGLVGQLL MQPVLVAQGT PGMAQAQAQA QAQAQAQAQA PAPAPAPAPA PATASASAGT 600
TNTATTAGPA PGGPAQPPPP QPSAADLQFS QLLGNLLGPA GPGAGGPGMA SPTITVAMPG 660
VPAFLQGMTD FLQASQTAPP PPPPPPPPPP APEQQSTPPP GSPSGGTASP GGLGPESLPP 720
EFFTSVVQGV LSSLLGSLGA RAGSSESIAA FIQRLSGSSN IFEPGADGAL GFFGALLSLL 780
CQNFSMVDVV MLLHGHFQPL QRLQPQLRSF FHQHYLGGQE PTPSNIRMAT HTLITGLEEY 840
VRESFSLVQV QPGVDIIRTN LEFLQEQFNS IAAHVLHCTD SGFGARLLEL CNQGLFECLA 900
LNLHCLGGQQ MELAAVINGR IRRMSRGVNP SLVSWLTTMM GLRLQVVLEH MPVGPDAILR 960
YVRRVGDPPQ TLPEEPMEVQ GAERTSPEPQ RENASPAPGT TAEEAMSRGP PPAPEGGSRD 1020
EQDGASADAE PWAAAVPPEW VPIIQQDIQS QRKVKPQPPL SDAYLSGMPA KRRKTMQGEG 1080
PQLLLSEAVS RAAKAAGARP LTSPESLSRD LEAPEVQESY RQQLRSDIQK RLQEDPNYSP 1140
QRFPNAHRAF ADDP 1154 
Gene Ontology
 GO:0071818; C:BAT3 complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0031593; F:polyubiquitin binding; IDA:UniProtKB.
 GO:0070628; F:proteasome binding; IDA:UniProtKB.
 GO:0043022; F:ribosome binding; ISS:UniProtKB.
 GO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:UniProtKB.
 GO:0007420; P:brain development; IMP:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; IMP:UniProtKB.
 GO:0018393; P:internal peptidyl-lysine acetylation; ISS:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IMP:UniProtKB.
 GO:0001822; P:kidney development; IMP:UniProtKB.
 GO:0030324; P:lung development; IMP:UniProtKB.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0050821; P:protein stabilization; IMP:UniProtKB.
 GO:0042981; P:regulation of apoptotic process; IMP:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0007283; P:spermatogenesis; IMP:UniProtKB.
 GO:0007130; P:synaptonemal complex assembly; IMP:UniProtKB.
 GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
 GO:0006810; P:transport; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB. 
Interpro
 IPR021925; DUF3538.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12057; DUF3538
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS