CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023236
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein kinase C and casein kinase substrate in neurons protein 2 
Protein Synonyms/Alias
 Syndapin 2; Syndapin-II 
Gene Name
 PACSIN2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53HERARIEKAYAQQLTubiquitination[1]
72RWRQLVEKGPQYGTVubiquitination[1]
111LMNDDFEKIKNWQKEubiquitination[1]
117EKIKNWQKEAFHKQMacetylation[2]
129KQMMGGFKETKEAEDubiquitination[1]
177ISREANSKADPSLNPubiquitination[3]
213EKYEKSLKELDQGTPubiquitination[4]
264LSNVAGYKAIYHDLEubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Plays a role in the formation of normal, flask-shaped caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis. Plays a role in intracellular vesicle-mediated transport. Binds to membranes via its F-BAR domain and promotes membrane tubulation. Plays a role in the internalization of cell-surface receptors. Is not required for internalization of EGFR after EGF stimulus, but contributes to the internalization of EGFR in the absence of EGF stimulus. 
Sequence Annotation
 DOMAIN 11 75 FCH.
 DOMAIN 426 486 SH3.
 REGION 1 306 F-BAR domain.  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endocytosis; Endosome; Lipid-binding; Membrane; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCSD LMNCLHERAR IEKAYAQQLT 60
EWARRWRQLV EKGPQYGTVE KAWMAFMSEA ERVSELHLEV KASLMNDDFE KIKNWQKEAF 120
HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK EVEAAKKAHH AACKEEKLAI SREANSKADP 180
SLNPEQLKKL QDKIEKCKQD VLKTKEKYEK SLKELDQGTP QYMENMEQVF EQCQQFEEKR 240
LRFFREVLLE VQKHLDLSNV AGYKAIYHDL EQSIRAADAV EDLRWFRANH GPGMAMNWPQ 300
FEEWSADLNR TLSRREKKKA TDGVTLTGIN QTGDQSLPSK PSSTLNVPSN PAQSAQSQSS 360
YNPFEDEDDT GSTVSEKDDT KAKNVSSYEK TQSYPTDWSD DESNNPFSST DANGDSNPFD 420
DDATSGTEVR VRALYDYEGQ EHDELSFKAG DELTKMEDED EQGWCKGRLD NGQVGLYPAN 480
YVEAIQ 486 
Gene Ontology
 GO:0005901; C:caveola; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0019898; C:extrinsic to membrane; ISS:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0005215; F:transporter activity; TAS:ProtInc.
 GO:0030036; P:actin cytoskeleton organization; TAS:ProtInc.
 GO:0070836; P:caveola assembly; IMP:UniProtKB.
 GO:0072584; P:caveolin-mediated endocytosis; IMP:UniProtKB.
 GO:0048858; P:cell projection morphogenesis; ISS:UniProtKB.
 GO:0097320; P:membrane tubulation; ISS:UniProtKB.
 GO:0045806; P:negative regulation of endocytosis; IEA:Compara. 
Interpro
 IPR001060; FCH_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00611; FCH
 PF00018; SH3_1 
SMART
 SM00055; FCH
 SM00326; SH3 
PROSITE
 PS50133; FCH
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.