CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005352
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM3 
Protein Synonyms/Alias
 DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3 
Gene Name
 Mcm3 
Gene Synonyms/Alias
 Mcmd; Mcmd3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
293KFSKTRSKDVFEQLAacetylation[1]
413VCIDEFDKMSDMDRTacetylation[2]
435QGRVTIAKAGIHARLacetylation[2]
547QDTRIYEKHDSLLHGacetylation[1, 2]
697KRRKTHAKDGESYDPacetylation[1, 3]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. 
Sequence Annotation
 DOMAIN 295 502 MCM.
 NP_BIND 345 352 ATP (Potential).
 MOTIF 477 480 Arginine finger.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 160 160 Phosphoserine (By similarity).
 MOD_RES 668 668 Phosphoserine.
 MOD_RES 672 672 Phosphoserine.
 MOD_RES 681 681 Phosphoserine (By similarity).
 MOD_RES 705 705 Phosphotyrosine (By similarity).
 MOD_RES 708 708 Phosphoserine (By similarity).
 MOD_RES 719 719 Phosphothreonine.
 MOD_RES 722 722 Phosphothreonine (By similarity).  
Keyword
 Acetylation; ATP-binding; Cell cycle; Complete proteome; DNA replication; DNA-binding; Glycoprotein; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 812 AA 
Protein Sequence
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQNKVRELI SDNQYRLIVS VNDLRRKNEK 60
RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYI GLEGSFGSKH VSPRTLTSCF 120
LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA TKKTIERRYS DLTTLVAFPS SSVYPTKDEE 180
NNPLETEYGL SVYKDHQTIT IQEMPEKAPA GQLPRSVDVI LDDDLVDKVK PGDRIQVVGT 240
YRCLPGKKGC YTSGTFRTVL IACNVKQMSK DIQPAFSADD IAKIKKFSKT RSKDVFEQLA 300
RSLAPSIHGH DYVKKAILCL LLGGVERELE NGSHIRGDIN ILLIGDPSVA KSQLLRYVLC 360
TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL ADRGVVCIDE FDKMSDMDRT 420
AIHEVMEQGR VTIAKAGIHA RLNARCSVLA AANPVYGRYD QYKTPMENIG LQDSLLSRFD 480
LLFIMLDQMD PEQDREISDH VLRMHQYRAP GEQDGDALPL GSSVDILATD DPDFTQDDQQ 540
DTRIYEKHDS LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP TLTQESAAYI AEEYSRLRSQ 600
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ YAYFKKVLEK 660
EKKRKKASED ESDLEDEEEK SQEDTEQKRK RRKTHAKDGE SYDPYDFSEA ETQMPQVHTP 720
KTDDSQEKTD DSQETQDSQK VELSEPRLKA FKAALLEVFQ EAHEQSVGML HLTESINRNR 780
EEPFSSEEIQ ACLSRMQDDN QVMVSEGIVF LI 812 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0042555; C:MCM complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0032508; P:DNA duplex unwinding; IEA:GOC.
 GO:0006270; P:DNA replication initiation; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR008046; Mcm3.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00493; MCM 
SMART
 SM00382; AAA
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01659; MCMPROTEIN3.