CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016266
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin repeat domain-containing protein 46 
Protein Synonyms/Alias
 Ankyrin repeat small protein; ANK-S 
Gene Name
 ANKRD46 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116ATPLVLAKRRGVNKDubiquitination[1]
137SLEEQEVKGFNRGTHubiquitination[1, 2, 3, 4, 5, 6]
146FNRGTHSKLETMQTAubiquitination[3]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 REPEAT 11 40 ANK 1.
 REPEAT 44 73 ANK 2.
 REPEAT 77 103 ANK 3.
 REPEAT 107 138 ANK 4.  
Keyword
 Alternative splicing; ANK repeat; Complete proteome; Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 232 AA 
Protein Sequence
MSYVFVNDSS QTNVPLLQAC IDGDFNYSKR LLESGFDPNI RDSRGRTGLH LAAARGNVDI 60
CQLLHKFGAD LLATDYQGNT ALHLCGHVDT IQFLVSNGLK IDICNHQGAT PLVLAKRRGV 120
NKDVIRLLES LEEQEVKGFN RGTHSKLETM QTAESESAME SHSLLNPNLQ QGEGVLSSFR 180
TTWQEFVEDL GFWRVLLLIF VIALLSLGIA YYRRTLRLGS FARQDRSRIQ AI 232 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom. 
Pfam
 PF12796; Ank_2 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS