CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045289
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Bifunctional polynucleotide phosphatase/kinase 
Protein Synonyms/Alias
  
Gene Name
 PNKP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
162VFTAAGVKPQGKVAGubiquitination[1]
183LITTRSGKVFPTGPSacetylation[2]
183LITTRSGKVFPTGPSubiquitination[1]
226QMSIGRGKLPAEEFKacetylation[2]
226QMSIGRGKLPAEEFKubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MGEVEAPGRL WLESPPGGAP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRTQ VELVADPETR 60
TVAVKQLGVN PSTTGTQELK PGLEGSLGVG DTLYLVNGLH PLTLRWEETR TPESQPDTPP 120
GTPLVSQDEK RDAELPKKRM RKSNPGWENL EKLLVFTAAG VKPQGKVAGF DLDGTLITTR 180
SGKVFPTGPS DWRILYPEIP RKLRELEAEG YKLVIFTNQM SIGRGKLPAE EFKAKVEAVV 240
EKLGVPFQVL VATHAGLYRK PVTGMWDHLQ EQANDGTPIS IGDSIFVGDA AGRPANWAPG 300
RKKKDFSCAD RLRTVSRSGP LCLPESRALL SASPEVVVAV GFPGAGKSTF LKKHLVSAGY 360
VHVNRDTLGS WQRCVTTCET ALKQGKRVAI DNTNPDAASR ARYVQCARAA GVPCRCFLFT 420
ATLEQARHNN RFREMTDSSH IPVSDMVMYG YRKQFEAPTL AEGFSAILEI PFRLWVEPRL 480
GRLYCQFSEG 490 
Gene Ontology
  
Interpro
 IPR000253; FHA_dom.
 IPR023214; HAD-like_dom.
 IPR006549; HAD-SF_hydro_IIIA.
 IPR027417; P-loop_NTPase.
 IPR013954; PNK3P.
 IPR015636; PNK_3Pase_euk.
 IPR006550; PNK_3Pase_met.
 IPR006551; Polynucleotide_phosphatase.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF08645; PNK3P 
SMART
  
PROSITE
  
PRINTS