CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000261
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Segment polarity protein dishevelled homolog DVL-2 
Protein Synonyms/Alias
 Dishevelled-2; DSH homolog 2 
Gene Name
 DVL2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58AGAKYFFKSMDQDFGubiquitination[1]
301IYIGSIMKGGAVAADubiquitination[2]
343VLRDIVHKPGPIVLTubiquitination[1, 3]
353PIVLTVAKCWDPSPQubiquitination[1]
428TDMASVTKAMAAPESubiquitination[1]
477PERREARKYASGLLKubiquitination[1, 3]
484KYASGLLKAGLIRHTubiquitination[1, 2, 4, 5]
615EERAPESKSGSGSESubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes (By similarity). 
Sequence Annotation
 DOMAIN 11 93 DIX.
 DOMAIN 267 339 PDZ.
 DOMAIN 433 507 DEP.
 MOD_RES 211 211 Phosphoserine.  
Keyword
 3D-structure; Cell membrane; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Developmental protein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 736 AA 
Protein Sequence
MAGSSTGGGG VGETKVIYHL DEEETPYLVK IPVPAERITL GDFKSVLQRP AGAKYFFKSM 60
DQDFGVVKEE ISDDNARLPC FNGRVVSWLV SSDNPQPEMA PPVHEPRAEL APPAPPLPPL 120
PPERTSGIGD SRPPSFHPNV SSSHENLEPE TETESVVSLR RERPRRRDSS EHGAGGHRTG 180
GPSRLERHLA GYESSSTLMT SELESTSLGD SDEEDTMSRF SSSTEQSSAS RLLKRHRRRR 240
KQRPPRLERT SSFSSVTDST MSLNIITVTL NMEKYNFLGI SIVGQSNERG DGGIYIGSIM 300
KGGAVAADGR IEPGDMLLQV NDMNFENMSN DDAVRVLRDI VHKPGPIVLT VAKCWDPSPQ 360
AYFTLPRNEP IQPIDPAAWV SHSAALTGTF PAYPGSSSMS TITSGSSLPD GCEGRGLSVH 420
TDMASVTKAM AAPESGLEVR DRMWLKITIP NAFLGSDVVD WLYHHVEGFP ERREARKYAS 480
GLLKAGLIRH TVNKITFSEQ CYYVFGDLSG GCESYLVNLS LNDNDGSSGA SDQDTLAPLP 540
GATPWPLLPT FSYQYPAPHP YSPQPPPYHE LSSYTYGGGS ASSQHSEGSR SSGSTRSDGG 600
AGRTGRPEER APESKSGSGS ESEPSSRGGS LRRGGEASGT SDGGPPPSRG STGGAPNLRA 660
HPGLHPYGPP PGMALPYNPM MVVMMPPPPP PVPPAVQPPG APPVRDLGSV PPELTASRQS 720
FHMAMGNPSE FFVDVM 736 
Gene Ontology
 GO:0045177; C:apical part of cell; IBA:RefGenome.
 GO:0005938; C:cell cortex; IBA:RefGenome.
 GO:0030136; C:clathrin-coated vesicle; IBA:RefGenome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IBA:RefGenome.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0044340; P:canonical Wnt receptor signaling pathway involved in regulation of cell proliferation; IDA:BHF-UCL.
 GO:0021535; P:cell migration in hindbrain; IBA:RefGenome.
 GO:0034613; P:cellular protein localization; IEA:Compara.
 GO:0090103; P:cochlea morphogenesis; IBA:RefGenome.
 GO:0060026; P:convergent extension; IBA:RefGenome.
 GO:0022007; P:convergent extension involved in neural plate elongation; IEA:Compara.
 GO:0035329; P:hippo signaling cascade; TAS:Reactome.
 GO:0001843; P:neural tube closure; ISS:BHF-UCL.
 GO:0003151; P:outflow tract morphogenesis; ISS:BHF-UCL.
 GO:0090179; P:planar cell polarity pathway involved in neural tube closure; IBA:RefGenome.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0043507; P:positive regulation of JUN kinase activity; IDA:BHF-UCL.
 GO:0061098; P:positive regulation of protein tyrosine kinase activity; IEA:Compara.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0007379; P:segment specification; ISS:BHF-UCL.
 GO:0006366; P:transcription from RNA polymerase II promoter; IDA:UniProtKB. 
Interpro
 IPR000591; DEP_dom.
 IPR008341; Dishevelled_2.
 IPR024580; Dishevelled_C-dom.
 IPR008339; Dishevelled_fam.
 IPR003351; Dishevelled_protein_dom.
 IPR001158; DIX.
 IPR015506; Dsh/Dvl-rel.
 IPR001478; PDZ.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00610; DEP
 PF02377; Dishevelled
 PF00778; DIX
 PF12316; Dsh_C
 PF00595; PDZ 
SMART
 SM00021; DAX
 SM00049; DEP
 SM00228; PDZ 
PROSITE
 PS50186; DEP
 PS50841; DIX
 PS50106; PDZ 
PRINTS
 PR01760; DISHEVELLED.
 PR01762; DISHEVELLED2.