CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013106
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase DTX3L 
Protein Synonyms/Alias
 Protein deltex-3-like 
Gene Name
 Dtx3l 
Gene Synonyms/Alias
 Bbap 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
340CFPKLLIKGQGRTLTubiquitination[1]
416TRGKVQEKGQKTCILacetylation[2]
473RARLHNTKFADNFKKubiquitination[1]
736YPDPDYLKRVKEELKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post- translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation (By similarity). In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (By similarity). 
Sequence Annotation
 ZN_FING 569 608 RING-type.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 9 9 Phosphoserine (By similarity).
 MOD_RES 215 215 Phosphoserine (By similarity).
 MOD_RES 536 536 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; DNA damage; DNA repair; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 748 AA 
Protein Sequence
MASSPDPPSP LLVRLRESIP KAHRKLEIYF QSRASGGGEC SVQPVGPSAP DTYEVKFLKK 60
ADKEKVLKKS EHEMLVHNKP VTIVLETTKK PVEDLRPRLP SLTQPVETPS SRPPSLTGSL 120
DEALCDDIHP QDGLVSNSVD SVVQKIFLAV TAELNCDLLS KEQRASITTV CPHIIKSMEG 180
SDGIKKVCGN FKDIEKIHHF LSEQLLEREQ KRKGSEQKRK CAPQKHTPPD VEREPPDQSS 240
IQVPVLLLEY FKHVNPGRLE FIEYKFGVNI EIQASSPNMV TVGFTSSPFG NVEEASQSFV 300
RDFQKCSQSL KQDCISLEEH QRAKEVRQEL SRCFPKLLIK GQGRTLTLLG SPADISAATE 360
KVSQGLGLRP VKITASGYTT GIEVDSTRFK LLEPELLQEI SEIEQKFNTR GKVQEKGQKT 420
CILFVPKDKD LDLSVQSYTG FTDAFQRATW QLRTEVLSLK GLGKERARLH NTKFADNFKK 480
EHPNVHFVTS QESVTLTGLP HHLAQAMQYV SKRMGLAPSS GEKLAMDQET PMEISSSDPH 540
GDQQENAALP APRGTSSSPA ASKGTEDYCV ICMDTISNKH VLPKCKHEFC TSCISKAMLI 600
KPVCPVCLTS YGIQKGNQPE GTMSYSTQKG SLPGYEGCGT IVINYEIKDG IQTKEHPNPG 660
KAYHGTRRTA YLPDNTEGRK VLDLLHEAFK HRLTFTIGYS RATGVSDVIT WNDIHHKTSK 720
FGGPANFGYP DPDYLKRVKE ELKAKGIE 748 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0042393; F:histone binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006302; P:double-strand break repair; ISS:UniProtKB.
 GO:0010390; P:histone monoubiquitination; ISS:UniProtKB. 
Interpro
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
  
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS