CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032403
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 10 kDa heat shock protein, mitochondrial 
Protein Synonyms/Alias
 Heat shock 10kDa protein 1 (Chaperonin 10), isoform CRA_b 
Gene Name
 HSPE1 
Gene Synonyms/Alias
 hCG_21429 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAGQAFRKFLPLFDRacetylation[1, 2]
8MAGQAFRKFLPLFDRubiquitination[3]
28SAAETVTKGGIMLPEubiquitination[2, 3, 4, 5]
36GGIMLPEKSQGKVLQubiquitination[2, 5, 6]
40LPEKSQGKVLQATVVacetylation[1, 7]
40LPEKSQGKVLQATVVubiquitination[3]
56VGSGSKGKGGEIQPVacetylation[1]
56VGSGSKGKGGEIQPVphosphoglycerylation[8]
56VGSGSKGKGGEIQPVubiquitination[2, 5]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237
Functional Description
  
Sequence Annotation
  
Keyword
 Chaperone; Complete proteome; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 101 AA 
Protein Sequence
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI 60
QPVSVKVGDK VLLPEYGGTK VVLDDKVCKL NNSKKKSDIC N 101 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR020818; Chaperonin_Cpn10.
 IPR018369; Chaprnonin_Cpn10_CS.
 IPR011032; GroES-like. 
Pfam
 PF00166; Cpn10 
SMART
 SM00883; Cpn10 
PROSITE
 PS00681; CHAPERONINS_CPN10 
PRINTS
 PR00297; CHAPERONIN10.