CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021077
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 EH domain-containing protein 4 
Protein Synonyms/Alias
 PAST homolog 2; mPAST2 
Gene Name
 Ehd4 
Gene Synonyms/Alias
 Past2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
35LRSLYQRKVLPLEEAphosphoglycerylation[1]
35LRSLYQRKVLPLEEAubiquitination[2]
61EDADFENKPMILLVGubiquitination[2]
150QLPNQVLKSISIIDSubiquitination[2]
165PGILSGEKQRISRGYubiquitination[2]
223KIRVVLNKADQVDTQubiquitination[2]
291DIQSLPQKAAVRKLNubiquitination[2]
296PQKAAVRKLNDLIKRubiquitination[2]
302RKLNDLIKRARLAKVubiquitination[2]
360AGDFPEVKAMQEQLEubiquitination[2]
478KKEMVTSKLPNSVLGubiquitination[2]
486LPNSVLGKIWKLADCubiquitination[2]
Reference
 [1] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Plays a role in early endosomal transport. 
Sequence Annotation
 DOMAIN 447 535 EH.
 DOMAIN 479 514 EF-hand.
 NP_BIND 68 75 ATP (By similarity).
 BINDING 223 223 ATP (By similarity).
 BINDING 261 261 ATP (By similarity).
 MOD_RES 451 451 Phosphotyrosine (By similarity).
 MOD_RES 459 459 Phosphoserine.  
Keyword
 ATP-binding; Calcium; Cell membrane; Complete proteome; Endosome; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 541 AA 
Protein Sequence
MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS PALEDADFEN 60
KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD 120
PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISIIDSPGI LSGEKQRISR GYDFCQVLQW 180
FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM 240
WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 300
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ ISAGDFPEVK 360
AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI SQEEMNMPTQ MVQGGAFDGT 420
TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP 480
NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA 540
D 541 
Gene Ontology
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0071363; P:cellular response to growth factor stimulus; IEA:Compara.
 GO:0032456; P:endocytic recycling; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0006907; P:pinocytosis; IEA:Compara.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0051260; P:protein homooligomerization; ISS:UniProtKB.
 GO:0030100; P:regulation of endocytosis; IEA:Compara. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS